LOCUS       sequence-134431-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retrovirus construct for stable expressing mt-mKeima.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Lazarou M, Sliter DA, Kane LA, Sarraf SA, Wang C, Burman JL, Sideris
            DP, Fogel AI, Youle RJ
  TITLE     The ubiquitin kinase PINK1 recruits autophagy receptors to induce 
            mitophagy.
  JOURNAL   Nature. 2015 Aug 20;524(7565):309-14. doi: 10.1038/nature14893. Epub
            2015 Aug 12.
  PUBMED    26266977
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14893"; journalName: "Nature"; date: "2015-08-20- 
            20"; volume: "524"; issue: "7565"; pages: "309-14"
COMMENT     Sequence Label: pCHAC-mt-mKeima
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..119
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             153..227
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 tcctctagac tgccggatcc acgcgtatgc atctcgaggc caccatgtcc gtcctgacgc
       61 cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaga
      121 tccattcgtt gccgccggag gggaagcttg ggatgtccgt cctgacgccg ctgctgctgc
      181 ggggcttgac aggctcggcc cggcggctcc cagtgccgcg cgccaagatc cattcgttgc
      241 cgccggaggg gaagctcggg ggatccgcca tggtgagcgt gatcgccaag cagatgacct
      301 acaaggtgta catgagcggc accgtgaacg gccactactt cgaggtggag ggcgacggca
      361 agggcaagcc ctacgagggc gagcagaccg tgaagctgac cgtgaccaag ggtggccccc
      421 tgcccttcgc ctgggacatc ctgagccccc agctccagta cggcagcatc cccttcacca
      481 agtaccccga ggacatcccc gactacttca agcagagctt ccccgagggc tacacctggg
      541 agcgcagcat gaacttcgag gacggcgccg tgtgcaccgt gagcaacgac agcagcatcc
      601 agggcaactg cttcatctac aacgtgaaga tcagcggcga gaacttcccc cccaacggcc
      661 ccgtgatgca gaagaagacc cagggctggg agcccagcac cgagcgcctg ttcgcccgcg
      721 acggaatgct gatcggcaac gactacatgg ccctgaagct ggagggcggc ggccactacc
      781 tgtgcgagtt caagagcacc tacaaggcca agaagcccgt gaggatgccc ggccgccacg
      841 agatcgaccg caagctggac gtgaccagcc acaaccgcga ctaca
//
