LOCUS       sequence-134433-        5164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C. elegans optimized BirA under control of the ubiquitous 
            rps-27 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pMB37
FEATURES             Location/Qualifiers
     source          1..5164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             866..895
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             902..931
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2604..2626)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2726..2745
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2823..2841)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2909..3013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3014..3082,3083..3874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3232..3251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4045..4633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4534..4553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4787..4804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4921..4942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4957..4962,4963..4980,4981..4987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4995..5011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5000..5022
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5019..5035
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5019..5035
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagttcaa tcggtttttc cttgcttgcg cccaattctg atggttcttc
       61 catttctaaa ttttagttta ataaaaaaat tttaaaataa tacgagtttt gagactaact
      121 ttcaataaaa accaaaaaaa attgcttcaa aaaagagttc gcgccgattt acgataaaca
      181 cagcgcgcta cgacactccg aaaaccgcgc cgcaccatta cattctccgc cccaccgcac
      241 ggtttgcgga aggcgatgaa attgctgggc ctaaaatttt aacccttttt ttcccgtttt
      301 tcaactgttt tcccgatttt ttcgatattt ttttcaggtt tttagttaga aaagtatata
      361 aaaattgcat tatttgtagt tttcgctcgt aatttgtgat agaaaacggc caatttgcct
      421 agatttgtga ttctaccaag tggaattttt gggaaaatat caatattcca cgatttttat
      481 tcaaatcata acgtttcgaa attatcagct taaataaaac taaaataaat aataaaatac
      541 taaaaaccct gaatttctcg gttctcggtt cagcttgagt tcgttctata cacgttatcg
      601 atttttgtca tttcgtttaa tcatgggttt tgattcattt taaatatgaa atttgattta
      661 ttcctcgttt cgaagttggt ttgtcttgtc cggccggtaa acaaatgtta ttaaaaaaat
      721 aataaatttc gaaatttttc gtaatgttcc gcaatcgtgt ttttccgttt atctcgtctt
      781 cccttctttc aaatatacta tttattttct caattcacat attttcagcc agccccgctc
      841 aacaagtgga atggtaccaa aaatggagca gaagctcatc tctgaggaag acctcggagg
      901 agagcagaaa ctcatctctg aagaggacct catgaaggat aatactgttc cattgaagtt
      961 gattgctttg cttgcaaatg gagaatttca ttctggagaa caacttggag agacgttggg
     1021 aatgtcgcgt gctgcaatta acaagcatat tcaaacagta agtttaaaca gttcggtact
     1081 aactaaccat acatatttaa attttcagtt gagagactgg ggagttgacg tttttacggt
     1141 tccaggaaag ggatattcgc ttccagagcc aattcagctc ttgaatgcaa agcaaatttt
     1201 gggacagctt gatggtggta gtgttgcagt acttccggtt atagatagta caaatcagta
     1261 cttgcttgat cgaataggag aattgaaatc aggagatgct tgcatcgctg agtatcagca
     1321 ggctggacga ggacgaagag gacgtaagtg gttctgtaag tttaaacata tatatactaa
     1381 ctaaccctga ttatttaaat tttcagcgcc tttcggagcc aatctctatt tgagtatgtt
     1441 ctggcgtctt gaacaaggtc cggccgctgc tatcggactt tcattggtta ttggaattgt
     1501 gatggcagaa gttctccgaa aacttggtgc agataaggtg agagtcaagt ggcctaacga
     1561 tctttatctt caagatagaa aattggccgg aatattggta agtttaaaca tatatatact
     1621 aactaaccct gattatttaa attttcaggt tgagcttact ggaaagacgg gagatgctgc
     1681 tcaaattgtg attggagctg gtatcaatat ggcaatgaga agagtagaag agtctgttgt
     1741 taatcaaggt tggatcacac ttcaagaggc aggaatcaac cttgatagaa atacgttggc
     1801 cgccatgctt atccgagaat tgcgagctgc actcgaactc ttcgaacaag aaggtcttgt
     1861 aagtttaaac atatatatac taactaaccc tgattattta aattttcagg ccccttacct
     1921 cagtagatgg gagaaacttg acaattttat caatcgacca gtgaagttga taattggaga
     1981 taaggaaatt tttggtatca gtcgaggaat tgataagcaa ggagctcttc tccttgagca
     2041 agatggaatc attaaaccat ggatgggtgg agagatttct ttgcgatctg ctgaaaagta
     2101 agaattcgct agccggccat acaagtaatc cggatgatcg acgccaacgt cgttgaattt
     2161 tcaaatttta aatactgaat atttgttttt tttcctatta tttatttatt ctctttgtgt
     2221 tttttttctt gctttctaaa aaattaattc aatccaaatc taaacatttt tttttctctt
     2281 tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat
     2341 cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa
     2401 aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg
     2461 gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa
     2521 taagcaaaga aattaggcga acgatgagaa ttgtcctcgc ttgggccctt tcgtctcgcg
     2581 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2641 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2701 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     2761 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     2821 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     2881 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2941 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3001 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3061 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3121 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3181 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3241 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3301 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3361 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3421 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3481 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3541 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3601 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3661 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3721 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3781 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3841 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3901 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3961 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4021 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4081 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4141 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4201 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4261 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4321 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4381 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     4441 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4501 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4561 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4621 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4681 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4741 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4801 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     4861 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     4921 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     4981 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5041 ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt
     5101 cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaatg
     5161 aaat
//
