LOCUS       sequence-134436-        7993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C. elegans optimized BirA under control of the intestinal 
            elt-2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pMB71
FEATURES             Location/Qualifiers
     source          1..7993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2353..2364
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3695..3724
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3731..3760
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5433..5455)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5555..5574
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5652..5670)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5738..5842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5843..5911,5912..6703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6061..6080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6874..7462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7363..7382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7616..7633
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7750..7771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7786..7791,7792..7809,7810..7816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7824..7840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7829..7851
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7848..7864
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7848..7864
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtaatt tcgaaatgta tgaactccaa ttcttgataa ccaattcctg
       61 actctgtaac tattattatt ataatcctat tacccacctt ttttcccatt ctgctcttag
      121 ttctccccca acccgtacat tttctcaact aatgaagaca atgagaaagt gagagagaat
      181 aagacgaact gaaaagaatg tgtaaacagt ttattgcctc tcgattgtac catctagtgt
      241 cttgtcgtgt gcataactac tgataacaga tcgaatgtga aagacccaat aaatgcttgg
      301 aaagtatcaa atatgtttag aaatagtatt tagagactta tgtgggacga aaaaacagga
      361 gcattaagaa tacagttgca aacattcatc aagttataat ctaataatat aaatccaaaa
      421 atgttaatga taaaaaatga tgatcaaagt aggaaattag tggcgagaaa acaataaatc
      481 atcagaaaat gactgatctt gagtagagtg ctttttttgt agatttctga ttctaaaact
      541 gaaaaaaaaa ctaaaaatgc tcgatgagtc atactaattg aaaaataatc agattgaaaa
      601 cgttaaaaat gtacttgcta acatacttcc atggttattt ttgaacacaa ataaagttat
      661 atacatcttt accggcacca gaagaaatca ccgctaacca atgttcagtt tttacctgaa
      721 tatccctgaa agtacaaaaa aatcaatgtt caatcaagtt tttgcatgcc ttcgtgtgat
      781 ggctcgcacc aagtattctg acagttataa aatgtttcct gtgatcatga atttgaaaaa
      841 aaaagaactg atgggaaaat gtgatattca taaatgaaat gtttaataaa caaaaaagaa
      901 ccgtcatcac aaatctactc tgaaacgttc acagttccta attgaccaat tcagaatttt
      961 ttgtactgac ttaataaata taaatttaca gttttaagaa acataaattg aattatataa
     1021 ataaaaaaaa aaaaactaaa atgaagacgt gatggaatgt ttcacgtatt aaaaaagctc
     1081 tcggaccgtt tgacatccag aaattatgtg aaagtgattt tctaaccctt tgacgtcaaa
     1141 aaagtttttc ttttttgaaa gatacgctgt ttagaaatac ttatacagct gattttagaa
     1201 tctccaaaaa accttcaatt acttaacaaa ctttctaaat aaagaatttt gattttgatt
     1261 ttctttgaac ttcaaacccc ccgttaaaaa accaaattaa aaaaaagata ccagattaat
     1321 tttaaagtga aaaatataaa tttattcccg actgttaaaa acggtttata aacggagtca
     1381 caacactcat atacaactat cgacaccaaa taaataactt cgaaagctaa ttgtgtcatt
     1441 tcggatatag agagtagtgt gagcagatgt gtttttgcag aactaaattg atgacactat
     1501 tatggaatat aatgaccaaa tgttaaatgt gttaaggttt gatatcaaaa cctgtatttt
     1561 ctttttatac agaattgata atgttatctt caattgattt ctacttctga gctacggcga
     1621 tacgaggacg cattctcaac gataatgttg ccattttgtc ctgataattt ttttactgat
     1681 tgtttcagaa cacccatagt ttttctctat taaacgttca tccttgactt ccccgagttt
     1741 gctggctgaa taggaaattt gaagacaaaa aggaaagaat cggctcaaac gtcatgcaac
     1801 tgataaggcg accgtacttc atttcaaaag aagctcactt actgagcgca aacattgaga
     1861 aatgagagca aaagaagtgg ttttacaaca tgctaatgtt tttagacctt gtacccaata
     1921 atattactgt agtatacagt tcggagagca tatggttgaa atcttgaaat accaatttat
     1981 cactagtttg attgtgttat cgatgtataa agatatattt tatcattttt gattattatc
     2041 tgatattgtg gggtgtgaag taatattatg tgcgtgtgtg gctgattatc gaaaaaaact
     2101 gaaaattatc aatttttcta caggttatct ttttttgttt tatttttcat tattgtattc
     2161 ttcatactcc ttatcctgcc cgaatcacaa aagttccaga caacttgaag gtcgcatcac
     2221 gttttgttta taagaatgtt gaactggtca gctttggaga cagaactgtc ctccaaatgt
     2281 tgaatatgtt tccgtaaaac acactgtgta acaaaaagcg taccctttga tccggtattc
     2341 ttgaaaaaaa acatagaagg gcgtactttt catatctcac aaatatgcgt tttttatttt
     2401 ataataatat catttattga taattatatg tatttgtatg ttttcttgtc tttaaaatta
     2461 tggtgtgaca tcacagttta aaaattatat gaaaactaat gagtgaaaag ttaaaaatgt
     2521 atagtttgcc gtattttctc cattagtatt gtattcaaaa cttatttcca attggtgagc
     2581 actataaact ttgtagggta attgaggttc aatactaatt tttgaatatc tagcttttga
     2641 caaattattc aagttttgac aatgatgcct tatcaaatta aaaaaaatta ccataaattc
     2701 ttgaaagctt ttgattttgt ttcactctgt gaaggatagt aatttatcaa cattttaact
     2761 agatacatct gttctggcaa taaattaaag caagactatt actatgtgca gtattaatag
     2821 ctgcattgca acttgtttga aagtttcagt gaaaacgcaa aaactcaatt taattttcaa
     2881 gtttgtttgc aaaattggat atatgtatcc atcaaaagtc tgatcgcctg ttttatctac
     2941 ttattgtctc ctcagttgat ttttttgtag ttattataaa cgctactttg aaaccaatat
     3001 tttcttcctc attccacttt tttcaattac caactaccgt acatgcaatg atgggcggac
     3061 ccgagtccca tgtttggctg gaagtgggtg gttgtctgcg tatatgagcg acagaggtcg
     3121 gggctgaaac tgataagaat agtcgacact aacgccataa tcgctagcca gccatcatgc
     3181 acaccgagct cggtgtgcac accatctttc ttttcaaacc aatacgcttt gtgccttcat
     3241 tgacaatttt ctttttgata aaatcagcct atctatactt cccaatcatt tttagtctta
     3301 tcgttgaaca gctatcgagg tgccactgtt ttcactgata tcttctaagt tactatggca
     3361 ttaacatctt tgtgttctta ttttatgggt tattttaatt aatttttgca gttaattttt
     3421 ggaatgagca agaaaatgtt aattgtaata tcttcgtctg aaaattgtct tcaaatagtt
     3481 ttaattttaa aggcagtatt taagaaaata cacttctcga agcatttttg aatttttgaa
     3541 tttttaaact gcttgatgtt ttaggtgcca ctgttttcat tgataagttt tgatgtataa
     3601 atgcttgatt ttcttggcat tctaataaaa tagaaactag aaaatagatt atagcccggg
     3661 tgccctatag tgagtcgtat tggtaccaaa aatggagcag aagctcatct ctgaggaaga
     3721 cctcggagga gagcagaaac tcatctctga agaggacctc atgaaggata atactgttcc
     3781 attgaagttg attgctttgc ttgcaaatgg agaatttcat tctggagaac aacttggaga
     3841 gacgttggga atgtcgcgtg ctgcaattaa caagcatatt caaacagtaa gtttaaacag
     3901 ttcggtacta actaaccata catatttaaa ttttcagttg agagactggg gagttgacgt
     3961 ttttacggtt ccaggaaagg gatattcgct tccagagcca attcagctct tgaatgcaaa
     4021 gcaaattttg ggacagcttg atggtggtag tgttgcagta cttccggtta tagatagtac
     4081 aaatcagtac ttgcttgatc gaataggaga attgaaatca ggagatgctt gcatcgctga
     4141 gtatcagcag gctggacgag gacgaagagg acgtaagtgg ttctgtaagt ttaaacatat
     4201 atatactaac taaccctgat tatttaaatt ttcagcgcct ttcggagcca atctctattt
     4261 gagtatgttc tggcgtcttg aacaaggtcc ggccgctgct atcggacttt cattggttat
     4321 tggaattgtg atggcagaag ttctccgaaa acttggtgca gataaggtga gagtcaagtg
     4381 gcctaacgat ctttatcttc aagatagaaa attggccgga atattggtaa gtttaaacat
     4441 atatatacta actaaccctg attatttaaa ttttcaggtt gagcttactg gaaagacggg
     4501 agatgctgct caaattgtga ttggagctgg tatcaatatg gcaatgagaa gagtagaaga
     4561 gtctgttgtt aatcaaggtt ggatcacact tcaagaggca ggaatcaacc ttgatagaaa
     4621 tacgttggcc gccatgctta tccgagaatt gcgagctgca ctcgaactct tcgaacaaga
     4681 aggtcttgta agtttaaaca tatatatact aactaaccct gattatttaa attttcaggc
     4741 cccttacctc agtagatggg agaaacttga caattttatc aatcgaccag tgaagttgat
     4801 aattggagat aaggaaattt ttggtatcag tcgaggaatt gataagcaag gagctcttct
     4861 ccttgagcaa gatggaatca ttaaaccatg gatgggtgga gagatttctt tgcgatctgc
     4921 tgaaaagtaa gaattcgcta gccggccata caagtaatcc ggatgatcga cgccaacgtc
     4981 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
     5041 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
     5101 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     5161 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     5221 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     5281 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact
     5341 agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct tgggcccttt
     5401 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5461 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5521 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5581 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5641 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5701 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5761 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5821 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5881 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5941 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6001 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6061 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6121 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6181 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6241 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6301 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6361 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6421 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6481 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6541 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6601 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6661 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6721 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6781 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6841 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6901 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6961 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7021 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7081 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7141 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7201 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7261 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7321 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7381 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7441 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7501 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7561 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7621 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7681 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7741 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7801 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     7861 tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa ctattctcat
     7921 ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata cgctaacaac
     7981 ttggaaatga aat
//
