LOCUS       sequence-134437-        5999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C. elegans optimized BirA under control of the seam and 
            hypodermis wrt-2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pMB73
FEATURES             Location/Qualifiers
     source          1..5999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1701..1730
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1737..1766
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3439..3461)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3561..3580
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3658..3676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3744..3848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3849..3917,3918..4709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4067..4086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4880..5468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5369..5388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5622..5639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5756..5777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5792..5797,5798..5815,5816..5822)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5830..5846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5835..5857
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5854..5870
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5854..5870
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcaggt cgactccacg taatttcaca acatccggga aaagaagtga
       61 aaaaaggact cgagcaactg tacaagaaaa ttgagaagaa cctggttgcc aattcatcac
      121 ttttgcaagt tgtatggaga gatatgcaag agcaatttgt caagcaaatt gcggaataca
      181 acaaactgat tctcacttgt tacccaggct cgaagattga gctcgaagtt tccactgaca
      241 ctgtacttca gttcttctca gaaattgctc aacaacacta atattacgac gaataatttt
      301 attgaatttt gttgttttaa ttcatttaaa atttagtttt aatttatcag aactctaata
      361 cttactgaac catgctttgt aatatattta tttatctatt ctgtaatagg tgaacttcgt
      421 tacattactg atgtctgtaa tataaattta cgggtgttcc ccattcgaaa atagtagtaa
      481 tgcttgcttt tttgaaaaat aaatgtcttg cttggtgact tttatttcac gtattttcaa
      541 atgctcacaa aaatttcacg gaactgactt cttggttaca catttgtaga aattttgact
      601 gaatgtttgc attgaactgg tcaacttaag aatctgacat gcaaatggtg tgttttctga
      661 agaacacact ttggcaactt ttaataaaaa aggggtgtaa cacgatttaa atctaataaa
      721 aacaacttct ataatgttat tccgtttata tctcacttta tgttttacat tttactctta
      781 tatacatttt ttgactcatt tatacagggt ggtccataat tatggaccac cctgtactta
      841 tatcttattt aaaactattt catgtaagaa ttgttttaac agcgcatttt ttttcatcac
      901 tcgatttaaa ggtaaaattg ataggccgca atctattttg gaaaacttca gaacaaaatt
      961 taacatttta agaggtaact gtgaaggttt ataagagaat tataaaccga tctaaccact
     1021 aaacatggta acgtagaaaa agtaaaatta ggggaggggg gatgtttatt tattcttggt
     1081 tttcaattct tatcagactc tgtaaagtaa aatgaacata acttgttcga atagtgtagg
     1141 aatttcaaac aagctacaaa tctagctcca catacgcgtg gaatagccgg tcataaatcg
     1201 tcttccgtta accgcatggc tagatccaca ccgattccat ttttcttgaa taaaacaata
     1261 gcttgatgaa aattgggtag ccaccgaaaa aactgattcg cgatccggat tattggtgac
     1321 aaacatacgg caagtgaaag tgttgcactg atagaaaccg atacgagaat atgccttttg
     1381 ggcccccccc cccccccccc tacggcttca ctcccggtgt tgtagatctg actttcactt
     1441 gccttatctc gaggaatctg gagaggagaa ggagggacaa ccgatccgcg cgcgcgtaac
     1501 aatacgggag atatagagga aaagcagcaa aaagacattt tgatgacctt attttcttgt
     1561 cgttgtcgag acggccattg tcccttccac gcccagcttt tcattgtagt tcaaatcgct
     1621 tctcattgac aatcaacctg ccaattgttt ctcggggatc cccgggtgcc ctatagtgag
     1681 tcgtattggt accaaaaatg gagcagaagc tcatctctga ggaagacctc ggaggagagc
     1741 agaaactcat ctctgaagag gacctcatga aggataatac tgttccattg aagttgattg
     1801 ctttgcttgc aaatggagaa tttcattctg gagaacaact tggagagacg ttgggaatgt
     1861 cgcgtgctgc aattaacaag catattcaaa cagtaagttt aaacagttcg gtactaacta
     1921 accatacata tttaaatttt cagttgagag actggggagt tgacgttttt acggttccag
     1981 gaaagggata ttcgcttcca gagccaattc agctcttgaa tgcaaagcaa attttgggac
     2041 agcttgatgg tggtagtgtt gcagtacttc cggttataga tagtacaaat cagtacttgc
     2101 ttgatcgaat aggagaattg aaatcaggag atgcttgcat cgctgagtat cagcaggctg
     2161 gacgaggacg aagaggacgt aagtggttct gtaagtttaa acatatatat actaactaac
     2221 cctgattatt taaattttca gcgcctttcg gagccaatct ctatttgagt atgttctggc
     2281 gtcttgaaca aggtccggcc gctgctatcg gactttcatt ggttattgga attgtgatgg
     2341 cagaagttct ccgaaaactt ggtgcagata aggtgagagt caagtggcct aacgatcttt
     2401 atcttcaaga tagaaaattg gccggaatat tggtaagttt aaacatatat atactaacta
     2461 accctgatta tttaaatttt caggttgagc ttactggaaa gacgggagat gctgctcaaa
     2521 ttgtgattgg agctggtatc aatatggcaa tgagaagagt agaagagtct gttgttaatc
     2581 aaggttggat cacacttcaa gaggcaggaa tcaaccttga tagaaatacg ttggccgcca
     2641 tgcttatccg agaattgcga gctgcactcg aactcttcga acaagaaggt cttgtaagtt
     2701 taaacatata tatactaact aaccctgatt atttaaattt tcaggcccct tacctcagta
     2761 gatgggagaa acttgacaat tttatcaatc gaccagtgaa gttgataatt ggagataagg
     2821 aaatttttgg tatcagtcga ggaattgata agcaaggagc tcttctcctt gagcaagatg
     2881 gaatcattaa accatggatg ggtggagaga tttctttgcg atctgctgaa aagtaagaat
     2941 tcgctagccg gccatacaag taatccggat gatcgacgcc aacgtcgttg aattttcaaa
     3001 ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt
     3061 ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac attttttttt ctctttccgt
     3121 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct
     3181 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat
     3241 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga
     3301 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc
     3361 aaagaaatta ggcgaacgat gagaattgtc ctcgcttggg ccctttcgtc tcgcgcgttt
     3421 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     3481 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     3541 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     3601 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc
     3661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3841 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3901 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3961 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4021 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4081 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4141 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4201 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4261 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4321 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4381 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4441 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4501 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4561 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4621 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4681 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4741 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4801 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     5281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5521 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5581 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5641 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5701 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5761 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     5821 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     5881 ccaagctgta agtttaaaca tgatcttact aactaactat tctcatttaa attttcagag
     5941 cttaaaaatg gctgaaatca ctcacaacga tggatacgct aacaacttgg aaatgaaat
//
