LOCUS       sequence-134456-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express tdTomato under EF-1 promoter when Cre is expressed by 
            another vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-DIO-tdTomato
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..61)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(112..145)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(273..296)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(471..490)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     740..758
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 attggatcct ctagagtcga ctccggaata acttcgtata ggatacttta tacgaagtta
       61 tgcagaatgg tagctggatt gtagctgcta ttagcaatat gaaacctctt aataacttcg
      121 tatagcatac attatacgaa gttatggcgc gccttattac ttgtacaagt aataaggcgc
      181 gccttattac ttgtacagct cgtccatgcc gtacaggaac aggtggtggc ggccctcgga
      241 gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggt
      301 gtccacgtag tagtagccgg gcagttgcac gggcttcttg gccatgtaga tggtcttgaa
      361 ctccaccagg tagtggccgc cgtccttcag cttcagggcc tggtggatct cgcccttcag
      421 cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg
      481 cattacgggg ccgtcggggg ggaagttggt gccgcgcatc ttcaccttgt agatcagcgt
      541 gccgtcctgc agggaggagt cctgggtcac ggtcaccaga ccgccgtcct cgaagttcat
      601 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtaatcgg ggatgtcggc
      661 ggggtgcttc acgtacgcct tggagccgta catgaactgg ggggacagga tgtcccaggc
      721 gaagggcagg gggccgccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
      781 gcggccctcg ccctcgccct cgatctcgaa
//
