LOCUS       sequence-134457-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Sharma N, Low SH, Misra S, Pallavi B, Weimbs T
  TITLE     Apical targeting of syntaxin 3 is essential for epithelial cell 
            polarity.
  JOURNAL   J Cell Biol. 2006 Jun 19. 173(6):937-48.
  PUBMED    16785322
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Jun 19. 173(6):937-48."
COMMENT     Sequence Label: Syn3D33A
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     56..80
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             803..814
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
       61 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      121 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc
      181 tcggatccac tagtccagtg tggtggaatt cctgggcttc aggatgaagg accgtctgga
      241 gcagctgaag gccaagcagc tgacacagga tgatgatact gatgcggttg agattgctat
      301 cgacaacacg gcttttatgg ccgagttctt ttctgagatt gaggaaactc ggcttaacat
      361 tgacaagatc tcagaacatg tagaggaggc taagaaactc tacagtatca ttctctctgc
      421 accgattcca gagccaaaaa ccaaggatga cctagagcag ctcacgactg agattaagaa
      481 aagggccaac aacgtccgga acaaactgaa gagcatggag aagcatattg aagaagatga
      541 ggtcaggtca tcggcagacc ttcggattcg gaaatcccag cactctgtcc tttctcggaa
      601 gtttgtggag gtgatgacca aatacaatga agctcaagtg gacttccgag aacgcagcaa
      661 agggcgaatc cagcggcagc tcgaaattac tggcaaaaag acaaccgatg aggagctgga
      721 ggagatgttg gagagtggca acccggccat cttcacttct gggatcattg actcacagat
      781 ttccaagcaa gccctcagtg agattgaggg acgacacaag gacattgtga ggctggagag
      841 cagcatcaag gagcttcacg acatgtttat ggacatcgcc atgctggtgg agaatcaggg
      901 tgagatgtta gataacatag agttgaatgt catgcacaca gtggaccacg tggagaaggc
      961 acgagatgaa a
//
