LOCUS       sequence-134458-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Sharma N, Low SH, Misra S, Pallavi B, Weimbs T
  TITLE     Apical targeting of syntaxin 3 is essential for epithelial cell 
            polarity.
  JOURNAL   J Cell Biol. 2006 Jun 19. 173(6):937-48.
  PUBMED    16785322
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Jun 19. 173(6):937-48."
COMMENT     Sequence Label: Syn3D33A
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    92..316
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     305..322
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(345..362)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(378..407)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(708..719)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtgggg atacccccta
       61 gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc
      121 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
      181 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
      241 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
      301 caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg atggtgatga
      361 tgaccggtat gcatattcag atcctcttct gagatgagtt tttgttcgaa tgggtgacct
      421 cgagcggccg cattcagccc aacggaaagt ccaataatca atgctaaaat gcccagcaac
      481 acaactacta gcacaatgat aattatcaat ttcttccggg cctgactctg gtatttcaca
      541 gcttttttcg tttcatctcg tgccttctcc acgtggtcca ctgtgtgcat gacattcaac
      601 tctatgttat ctaacatctc accctgattc tccaccagca tggcgatgtc cataaacatg
      661 tcgtgaagct ccttgatgct gctctccagc ctcacaatgt ccttgtgtcg tccctcaatc
      721 tcactgaggg cttgcttgga aatctgtgag tcaatgatcc cagaagtgaa gatggccggg
      781 ttgccactct ccaacatctc ctccagctcc tcatcggttg tctttttgcc agtaatttcg
      841 agctgccgct ggattcgccc tttgctgcgt tctcggaagt ccacttgagc ttcattgtat
      901 ttggtcatca cctccacaaa cttccgagaa aggacagagt gctggga
//
