LOCUS       sequence-134460-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express tdTomato under EF-1 promoter when Cre is expressed by 
            another vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-DIO-tdTomato
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..61)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(112..145)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(300..318)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcata acttcgtata gcatacatta tacgaagtta
       61 ttctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca aataacttcg
      121 tataggatac tttatacgaa gttatgctag cgctagcccc gggccaatgg tgagcaaggg
      181 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
      241 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      301 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
      361 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
      421 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
      481 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
      541 gcgcggcacc aacttccccc ccgacggccc cgtaatgca
//
