LOCUS       sequence-134472-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP under EF-1 promoter when Cre is expressed by another 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-DIO-copGFP
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..61)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(112..145)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attggatcct ctagagtcga ctccggaata acttcgtata ggatacttta tacgaagtta
       61 tgcagaatgg tagctggatt gtagctgcta ttagcaatat gaaacctctt aataacttcg
      121 tatagcatac attatacgaa gttatggcgc gccttagcga gatccggtgg agccgggtcc
      181 ggcggtgccg tccacggcag aattggacga ctgagcgcgg gatctggcga aggcaatggg
      241 ggtcttgaag gcgtgctggt actccacgat gcccagctcg gtgttgctgt gcagctcctc
      301 cacgcggcgg aaggcgaaca tggggccccc gttctgcagg atgctggggt ggatggcgct
      361 cttgaagtgc atgtggctgt ccaccacgaa gctgtagtag ccgccgtcgc gcaggctgaa
      421 ggtgcgggcg aagctgccca ccagcacgtt atcgcccatg gggtgcaggt gctccacggt
      481 ggcgttgctg cggatgatct tgtcggtgaa gatcacgctg tcctcgggga agccggtgcc
      541 caccaccttg aagtcgccga tcacgcggcc ggcctcgtag cggtagctga agctcacgtg
      601 cagcacgccg ccgtcctcgt acttctcgat gcgggtgttg gtgtagccgc cgttgttgat
      661 ggcgtgcagg aaggggttct cgtagccgct ggggtaggtg ccgaagtggt agaagccgta
      721 gcccatcacg tggctcagca ggtaggggct gaaggtcagg gcgcctttgg tgctcttcat
      781 cttgttggtc atgcggccct gcttgggggt gccctctccg ccgcccacca gctcgaactc
      841 cacgccgttc agg
//
