LOCUS       sequence-134475-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP under EF-1 promoter when Cre is expressed by another 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-DIO-copGFP
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(27..60)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(111..144)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             179..841
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
ORIGIN
        1 tccagaggtt gattgtcgac gaattcataa cttcgtatag catacattat acgaagttat
       61 tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggcaa ataacttcgt
      121 ataggatact ttatacgaag ttatgctagc caccatggag agcgacgaga gcggcctgcc
      181 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
      241 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
      301 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
      361 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
      421 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
      481 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
      541 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
      601 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
      661 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
      721 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
      781 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca ttgccttcgc
      841 cagatcccgc gctcagtcgt ccaattctgc cgtg
//
