LOCUS       sequence-134478-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK615
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..363
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             373..390
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(443..461)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      457..504
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      541..969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(628..647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     838..859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga gcgataaaat tattcacctg
       61 actgacgaca gttttgacac ggatgtactc aaagcggacg gggcgatcct cgtcgatttc
      121 tgggcagagt ggtgcggtcc gtgcaaaatg atcgccccga ttctggatga aatcgctgac
      181 gaatatcagg gcaaactaac cgttgcaaaa ctgaacatcg atcaaaaccc tggcactgcg
      241 ccgaaatatg gcatccgtgg tatcccgact ctgctgctgt tcaaaaacgg tgaagtggcg
      301 gcaaccaaag tgggtgcact gtctaaaggt cagttgaaag agttcctcga cgctaacctg
      361 gctagcctcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      421 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      481 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      541 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      601 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      661 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      721 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      781 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      841 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      901 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      961 acgcgaattt taacaaaata ttaacgctta ca
//
