LOCUS       sequence-134479-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK615
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(48..374)
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     RBS             382..404
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    419..443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(443..462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(444..462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(550..569)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        775..852
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(872..891)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgaggctagc caggttagcg
       61 tcgaggaact ctttcaactg acctttagac agtgcaccca ctttggttgc cgccacttca
      121 ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg cgcagtgcca
      181 gggttttgat cgatgttcag ttttgcaacg gttagtttgc cctgatattc gtcagcgatt
      241 tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca gaaatcgacg
      301 aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata
      361 attttatcgc tcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
      421 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      481 cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      541 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
      601 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
      661 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
      721 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc
      781 atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
      841 agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
      901 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa acgcgggaaa
      961 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     1021 gggcaacagt cgttgctgat gg
//
