LOCUS       sequence-134481-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli thioredoxin (EcTrxA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK645
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(48..374)
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     RBS             382..404
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(435..454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(436..454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagttacgc caggttagcg
       61 tcgaggaact ctttcaactg acctttagac agtgcaccca ctttggttgc cgccacttca
      121 ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg cgcagtgcca
      181 gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt
      241 tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca gaaatcgacg
      301 aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata
      361 attttatcgc tcatatgtat atctccttct taaagttaaa caaaattatt tctagaggga
      421 aaccgttgtg gtctccctat agtgagtcgt attaatttcg cgggatcgag atctcgggca
      481 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
      541 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
      601 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
      661 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
      721 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
      781 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
      841 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
      901 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat
      961 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     1021 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
     1081 cgctt
//
