LOCUS       sequence-134485-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Glover CP, Heywood DJ, Bienemann AS, Deuschle U, Kew JN, Uney JB
  TITLE     Adenoviral expression of CREB protects neurons from apoptotic and 
            excitotoxic stress.
  JOURNAL   Neuroreport. 2004 May 19;15(7):1171-5.
  PUBMED    15129168
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroreport."; date: "2004-05-19"; volume: "15(7)"; pages: "1171-5"
COMMENT     Sequence Label: pXCX.CMV.CREB.WPRE
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..777)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(104..124)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     719..747
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atccagaggt tgattgtcga cggatcctct agatgcatgc tcgagcggcc gccctcgagt
       61 ttatttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag
      121 gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
      181 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      241 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc
      301 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
      361 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      421 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      481 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
      541 tttcatatga tctgggtatc tcgcaaagca ttgaagacca tacgcaaaag tagtgacaag
      601 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc
      661 gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
      721 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tacccatggt
      781 ggcggcccgg tcgactctag ctagaggtac cacgcgtgcc atattatcat cgtgttttt
//
