LOCUS       sequence-134490-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 
            tag and Cys35>Ser mutant (AaTrxAH6-C35S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK581
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             376..393
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(446..464)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      460..507
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      544..999
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(631..650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     841..862
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga gtgaacatac gcttgctgtc
       61 agcgattcca gctttgatca agatgttctc aaggcctctg gccttgtgct ggtagatttc
      121 tgggcagaat ggtgcgggcc cagcaaaatg attggcccgg cattgggaga aattggcaag
      181 gaatttgccg gcaaggtaac ggttgccaag gtaaatattg atgacaaccc ggaaactcca
      241 aatgcgtatc aggtgcgtag cattccaacc cttatgctgg tgcgtgatgg caaggttata
      301 gataaaaaag tgggtgccct gcccaaaagc cagttgaagg catgggtaga aagcgctcag
      361 tcattagaac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      421 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      481 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      541 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      601 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      661 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      721 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      781 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      841 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      901 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      961 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttc
//
