LOCUS       sequence-134491-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 
            tag and Cys35>Ser mutant (AaTrxAH6-C35S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK581
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             385..407
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(438..457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(439..457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagttctaa tgactgagcg
       61 ctttctaccc atgccttcaa ctggcttttg ggcagggcac ccactttttt atctataacc
      121 ttgccatcac gcaccagcat aagggttgga atgctacgca cctgatacgc atttggagtt
      181 tccgggttgt catcaatatt taccttggca accgttacct tgccggcaaa ttccttgcca
      241 atttctccca atgccgggcc aatcattttg ctgggcccgc accattctgc ccagaaatct
      301 accagcacaa ggccagaggc cttgagaaca tcttgatcaa agctggaatc gctgacagca
      361 agcgtatgtt cactcatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      421 ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctcgg
      481 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
      541 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
      601 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
      661 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
      721 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
      781 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt
      841 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca
      901 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg
      961 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     1021 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggc
//
