LOCUS       sequence-134493-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a 
            C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK587
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             994..1011
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1064..1082)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1078..1125
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg ctgaaaccat tacaactgat
       61 ctgctcgtta ttggtgcggg acctgccgga tacaccgcag ctatttatgc ggcacgtgcc
      121 aacctttctc ccgttctggt cgctggcctt cagcctggcg ggcagctcac cattactaca
      181 gatgttgaaa actatccggg ttttgccaca gccatacagg ggccgtggct gatggaacaa
      241 atggcagccc aggcgcaaaa cgtgggcaca cgcattgaat acgacattat tacatctgtt
      301 aatttcaaag cgggttcacc ttttctgcta acaggtgatt ccggcacaac ctacaaggcc
      361 cgtgctgtta ttgttgccac aggcgcacag gcacgctggc ttggcctgcc aagtgaaaag
      421 catctgcaag gtgccggtgt ttctgctagc gccacctgcg atggcttttt ctaccgcggg
      481 aagaacgttg tggttgtcgg cggcggtaat actgcggttg aagaggccct gtatctcacc
      541 caccatgccg cgcatgtcac cttggtgcac cgccgagaca gcctgcgtgc tgaaaaaatt
      601 ctgcaagacc ggctttttgc caacccgaag gtttcggtga tctggaacag cgtggtgcaa
      661 gatattctgg ccgacggaac gccagaaacc gtatgcggtg tgcggctcaa gaatacgcag
      721 gataatagcg aacagactgt ctcgaccgat ggcgtattta ttgccattgg gcacgcccct
      781 aacacaggca tcttccgcaa ccagcttact ctggatgctg aagggtatat tgaaaccaca
      841 cccggcacca cacgtacatc tgtgcccgga gtctttgccg cgggggatgt gcaggacaaa
      901 acataccgcc aggccgtcac tgcggctggg actggctgta tggctgcgct agatgcggaa
      961 cggtatcttg ctggtttgag cggagaactc gagcaccacc accaccacca ctgagatccg
     1021 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     1081 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     1141 atatccggat tggc
//
