LOCUS       sequence-134502-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(1-393)
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatggaggag ccgcagtcag
      121 atcctagcgt cgagccccct ctgagtcagg aaacattttc agacctatgg aaactacttc
      181 ctgaaaacaa cgttctgtcc cccttgccgt cccaagcaat ggatgatttg atgctgtccc
      241 cggacgatat tgaacaatgg ttcaccgaag acccaggtcc agatgaagct cccagaatgc
      301 cagaggctgc tccccccgtg gcccctgcac cagcagctcc tacaccggcg gcccctgcac
      361 cagccccctc ctggcccctg tcatcttctg tcccttccca gaaaacctac cagggcagct
      421 acggtttccg tctgggcttc ttgcattctg ggacagccaa gtctgtgact tgcacgtact
      481 cccctgccct caacaagatg ttttgccaac tggccaagac ctgccctgtg cagctgtggg
      541 ttgattccac acccccgccc ggcacccgcg tccgcgccat ggccatctac aagcagtcac
      601 agcacatgac ggaggttgtg aggcgctgcc cccaccatga gcgctgctca gatagcgatg
      661 gtctggcccc tcctcagcat cttatccgag tggaaggaaa tttgcgtgtg gagtatttgg
      721 atgacagaaa cacttttcga catagtgtgg tggtgcccta tgagccgcct gaggttggct
      781 ctgactgtac caccatccac tacaactaca tgtgtaacag ttcctgcatg ggcggcatga
      841 accggaggcc catcctcacc atcatcacac tggaagactc cagtggtaat ctactgggac
      901 ggaacagctt tgaggtgcgt gtttgtgcct gtcctgggag agaccggcgc acagaggaag
      961 agaatctccg caagaaaggg gagc
//
