LOCUS       sequence-134518-        1926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Large fragment of split HRP and FRB fused to the extracellular 
            terminus of the ""pre mGRASP"" scaffold published by Magee, in which
            the extracellular domain of NRX is replaced by portions of CD4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: CAG sHRPa-FRB-pre mGRASP
FEATURES             Location/Qualifiers
     source          1..1926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1066..1089
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgccacctt ctactagtct tttgttgctt gctgcacttc tccccttcgc tcttcccgct
       61 tcagattgga agacaggaga agtcaccgct tccaccggtc agcttactcc aactttctac
      121 gataactctt gtcctaacgt gagcaacatc gtgcgagata ttatcgtgaa cgagttgcgg
      181 tctgatccga ggatagccgc ttcaattttg cggctgcatt tccacgattg ttttgtaaac
      241 ggctgtgatg cttccatcct cctcgacaat accacctctt tcaggactga aaaggatgcc
      301 ttcggtaacg cgaatagtgc tagagggttt tccgtgatcg atagaatgaa agccgctgtg
      361 gagagcgcct gccccgggac agttagttgt gcagaccttc tgaccatagc ggcccagcaa
      421 tccgtgacgc tggccggtgg ccccagctgg agggtccccc tgggaaggag ggactctctg
      481 caggcttttc tggacttggc aaacgcaaac ctgcctgctc ccttcttcac tcttccccag
      541 ctgaaggatt ccttcaggaa tgtcggcctg aaccgaagta gtgatttggt ggctctgtcc
      601 ggaggccaca cattcggcaa gagtcagtgc aggttcatca tggacagact gtataacttt
      661 tccaacaccg gcttgcctga ccctaccctg aacacaacat acttgcagac tctcagagga
      721 ctgtgtccgc tcaatggcaa gggctcgggc tcgacctcgg gctcgggcgg ctcgagcgtg
      781 gccatcctgt ggcacgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
      841 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
      901 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
      961 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcacccaa
     1021 gcctgggacc tctattatca tgtgttccga cgaatctcac aattggacta caaggatgac
     1081 gacgataagg tcgactttca gaaagctagc tctattgttt ataagaagga gggggaacag
     1141 gtggagttca gtttcccatt ggccttcaca gtcgagaagc tcacaggatc aggagagttg
     1201 tggtggcagg ccgaaagagc ctcctccagc aagagttgga tcacttttga tctgaaaaac
     1261 aaagaagtga gcgtgaagag agtcacacag gaccctaagc tgcagatggg caagaagttg
     1321 cctttgcact tgacactccc ccaagccctg ccccagtatg ctggctccgg gaacctcacc
     1381 ctcgccttgg aggctaagac cggaaagttg catcaggagg tcaacctcgt ggtgatgagg
     1441 gcaacacagc tgcagaagaa cctgacctgc gaagtgtggg gtcccacctc acctaaactg
     1501 atgctgtctc tgaagctgga gaataaggag gctaaagtca gtaaaagaga gaaggctgtg
     1561 tgggtcctta acccggaggc agggatgtgg cagtgtcttc ttagcgactc tggacaggtg
     1621 ctgctggaga gcaacatcaa ggtgcttcct acttggtcaa caccagtaca gccgatggcc
     1681 ctgatcgtac tcgggggagt cgccggcctg cttctgttta tcggcctggg catcttcttc
     1741 tgcgtacgct acaagtatcg caacagggat gagggatctt accacgttga cgaatctagg
     1801 aactatatca gtaacagcgc tcagagtaat ggcgccgtgg ttaaagagaa acagccctcc
     1861 agcgccaaat cagcaaacaa aaacaagaaa aacaaggata aagagtacta cgtagcggcc
     1921 gcgtga
//
