LOCUS       sequence-134520-        1470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Small fragment of split HRP and FKBP fused to the extracellular 
            terminus of the ""post mGRASP"" scaffold published by Magee, in 
            which the majority of the NLG1 extracellular domain is deleted..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: CAG sHRPb-FKBP-post mGRASP
FEATURES             Location/Qualifiers
     source          1..1470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             472..792
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             793..819
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggcacttc ccagatgcat gtggccaaat tatgtttgga gagctatgat ggcatgtgtg
       61 gtccacaggg gatccggtgc cccattgact ctctgcttgt tgggatgttt gctacagact
      121 tttcacgtac tctctcaaaa gaccggtaac ttgtctgcac tggttgactt tgacctgcgc
      181 accccaacca tcttcgacaa taagtattac gtcaatctgg aggaacagaa gggcctgatt
      241 cagtccgacc aggagctgtt cagttcccca gatgctaccg acactattcc tcttgtgcgc
      301 agcttcgcta atagcacaca aacctttttc aatgctttcg tggaagctat ggatcggatg
      361 ggaaatataa cgcccctgac gggtacgcag ggacagataa gacggaactg tagggtcgtg
      421 aacagcaact ctaagggctc gggctcgacc tcgggctcgg gcggctcgag cggagtgcag
      481 gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
      541 gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac
      601 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
      661 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
      721 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
      781 ctaaaactgg aatatccata tgatgttcca gattatgctc aattggtacc tcatctgcat
      841 aatctcaatg acatttctca gtatacctcg acaacaacta aagtgccatc cacggacatc
      901 actctcagac ctacaaggaa gaattccaca ccagtcacat cagcctttcc cactgccaaa
      961 caggatgatc ccaagcaaca accaagcccc ttctcggtgg atcagaggga ctactccaca
     1021 gagctaagtg tcactatcgc agtgggggcc tctctgctgt ttctcaacat cttggctttt
     1081 gcagccctgt actacaagaa ggataagagg agacatgatg tccaccggag gtgcagccct
     1141 cagcgcacga ccaccaacga cctaacccat gctccagaag aggaaattat gtctctccaa
     1201 atgaagcaca ctgacttgga tcacgagtgt gagtccatcc atccacatga ggtggttctt
     1261 cggaccgcct gtcccccaga ttatactcta gctatgagga ggtcacctga tgatattcca
     1321 ctaatgacac ctaacaccat cacaatgatt cccaacacta taccagggat tcagccctta
     1381 catacattca acacatttac tggaggacag aataatacac tgccccatcc ccacccacac
     1441 ccccattcac attcaacaac cagggtatag
//
