LOCUS       sequence-134524-       10485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large fragment of split HRP fused to the extracellular terminus of 
            neurexin3B. This is a lentiviral plasmid, but can also be used for 
            transfections. Synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: Synapsin sHRPa-NRX in FSW lentiviral vector
FEATURES             Location/Qualifiers
     source          1..10485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..3083
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3862..3885
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4777..4803
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4804..4830
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5177..5765
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5230..5250)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5648..5659)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5768..5784)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5769..5785)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6290..6470
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6496..6513)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6502..6726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6772..7200
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6859..6878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7069..7090
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7209..7229)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7214..7543
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7394..7529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7456..7475
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7591..7638
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7657..8031
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8161..8282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8198..8217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8252..8271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8331..8347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8331..8347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8344..8366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8355..8371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8379..8385,8386..8403,8404..8409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8424..8445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8562..8579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8733..9321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8813..8832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9492..10283,10284..10352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10115..10134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10353..10457)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta ataccgccat gcatctgcag agggccctgc gtatgagtgc
     2641 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
     2701 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
     2761 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     2821 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     2881 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     2941 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     3001 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     3061 tcgtgtcgtg cctgagagcg cagtctagac gccaccatgc acctgagaat ccacgcgaga
     3121 cggagccctc ctcgccggcc ggcctggacg cttgggatct ggttcctgtt ctggggatgt
     3181 atcgtcagct ctgtatggag taccggtcag cttactccaa ctttctacga taactcttgt
     3241 cctaacgtga gcaacatcgt gcgagatatt atcgtgaacg agttgcggtc tgatccgagg
     3301 atagccgctt caattttgcg gctgcatttc cacgattgtt ttgtaaacgg ctgtgatgct
     3361 tccatcctcc tcgacaatac cacctctttc aggactgaaa aggatgcctt cggtaacgcg
     3421 aatagtgcta gagggttttc cgtgatcgat agaatgaaag ccgctgtgga gagcgcctgc
     3481 cccgggacag ttagttgtgc agaccttctg accatagcgg cccagcaatc cgtgacgctg
     3541 gccggtggcc ccagctggag ggtccccctg ggaaggaggg actctctgca ggcttttctg
     3601 gacttggcaa acgcaaacct gcctgctccc ttcttcactc ttccccagct gaaggattcc
     3661 ttcaggaatg tcggcctgaa ccgaagtagt gatttggtgg ctctgtccgg aggccacaca
     3721 ttcggcaaga gtcagtgcag gttcatcatg gacagactgt ataacttttc caacaccggc
     3781 ttgcctgacc ctaccctgaa cacaacatac ttgcagactc tcagaggact gtgtccgctc
     3841 aatggcggct cgggctcggg cgactacaag gatgacgacg ataagcaatt gtcttctaat
     3901 gtagcttcct cctcctccac ctcttcctcg ccggggtctc actctcagca cgagcaccat
     3961 ttccatggca gcaagcatca ctcagtgcct atttctatct atcgttcccc tgtttccctt
     4021 cgaggaggac acgctggcgc tacgtacatc tttgggaaaa gtggtgggct tatcctctac
     4081 acctggccag ccaatgacag gcccagcacg cggtctgacc gccttgccgt gggcttcagc
     4141 accactgtga aggatggcat cttggtccgc atcgacagtg ctccaggact tggtgacttc
     4201 ctccagcttc acatagaaca ggggaaaatt ggagttgtct tcaacattgg cacagttgac
     4261 atctccatca aagaggagag aacccctgta aatgacggca aataccatgt ggtacgcttc
     4321 accaggaacg gcggcaacgc caccctgcag gtggacaact ggccagtgaa tgaacattat
     4381 cctacaggcc ggcagttaac catcttcaac actcaggcgc aaatagccat tggtggaaag
     4441 gacaaaggac gcctcttcca aggccaactc tctgggctct attatgatgg tttgaaagta
     4501 ctgaacatgg cggctgagaa caaccccaat attaaaatca atggaagtgt tcggctggtt
     4561 ggagaagtcc catcaatttt gggaacaaca cagacgacct ccatgccacc agaaatgtct
     4621 actactgtca tggaaaccac tactacaatg gcgactacca caacccgtaa gaatcgctct
     4681 acagccagca ttcagccaac atcagatgat cttgtttcat ctgctgaatg ttcaagtgat
     4741 gatgaagact ttgttgaatg tgagccgagt acagcatacc catacgatgt tccagattac
     4801 gcttatcctt acgacgtacc tgactatgct aaccccacgg agccgggaat cagacgggtt
     4861 ccgggggcct cagaggtgat ccgggagtcg agcagcacaa cagggatggt cgtcggcatt
     4921 gtggctgctg ccgccctctg catcttgatc ctcctgtacg ccatgtacaa gtacaggaac
     4981 agggacgagg ggtcctatca agtggacgag acgcggaact acatcagcaa ctccgcccag
     5041 agcaacggca cgctcatgaa ggagaagcag cagagctcga agagcggcca caagaaacag
     5101 aaaaacaagg acagggagta ttacgtgtag gtcgacggcg cgcccctgca gggaattcga
     5161 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5221 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5281 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5341 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5401 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5461 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5521 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5581 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5641 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5701 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5761 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     5821 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     5881 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5941 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6001 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     6061 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     6121 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     6181 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     6241 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     6301 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6361 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6421 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     6481 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6541 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6601 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6661 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6721 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     6781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6841 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6901 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6961 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7021 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7081 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7141 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7201 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7261 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7321 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7381 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7441 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7501 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7561 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7621 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7681 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7741 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     7801 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     7861 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     7921 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     7981 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     8041 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8101 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8161 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8221 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8281 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
