LOCUS       sequence-134525-        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Large fragment of split HRP fused to the extracellular terminus of 
            neurexin3B. This is a lentiviral plasmid, but can also be used for 
            transfections. Synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: Synapsin sHRPa-NRX in FSW lentiviral vector
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             766..789
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1681..1707
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1708..1734
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgcacctga gaatccacgc gagacggagc cctcctcgcc ggccggcctg gacgcttggg
       61 atctggttcc tgttctgggg atgtatcgtc agctctgtat ggagtaccgg tcagcttact
      121 ccaactttct acgataactc ttgtcctaac gtgagcaaca tcgtgcgaga tattatcgtg
      181 aacgagttgc ggtctgatcc gaggatagcc gcttcaattt tgcggctgca tttccacgat
      241 tgttttgtaa acggctgtga tgcttccatc ctcctcgaca ataccacctc tttcaggact
      301 gaaaaggatg ccttcggtaa cgcgaatagt gctagagggt tttccgtgat cgatagaatg
      361 aaagccgctg tggagagcgc ctgccccggg acagttagtt gtgcagacct tctgaccata
      421 gcggcccagc aatccgtgac gctggccggt ggccccagct ggagggtccc cctgggaagg
      481 agggactctc tgcaggcttt tctggacttg gcaaacgcaa acctgcctgc tcccttcttc
      541 actcttcccc agctgaagga ttccttcagg aatgtcggcc tgaaccgaag tagtgatttg
      601 gtggctctgt ccggaggcca cacattcggc aagagtcagt gcaggttcat catggacaga
      661 ctgtataact tttccaacac cggcttgcct gaccctaccc tgaacacaac atacttgcag
      721 actctcagag gactgtgtcc gctcaatggc ggctcgggct cgggcgacta caaggatgac
      781 gacgataagc aattgtcttc taatgtagct tcctcctcct ccacctcttc ctcgccgggg
      841 tctcactctc agcacgagca ccatttccat ggcagcaagc atcactcagt gcctatttct
      901 atctatcgtt cccctgtttc ccttcgagga ggacacgctg gcgctacgta catctttggg
      961 aaaagtggtg ggcttatcct ctacacctgg ccagccaatg acaggcccag cacgcggtct
     1021 gaccgccttg ccgtgggctt cagcaccact gtgaaggatg gcatcttggt ccgcatcgac
     1081 agtgctccag gacttggtga cttcctccag cttcacatag aacaggggaa aattggagtt
     1141 gtcttcaaca ttggcacagt tgacatctcc atcaaagagg agagaacccc tgtaaatgac
     1201 ggcaaatacc atgtggtacg cttcaccagg aacggcggca acgccaccct gcaggtggac
     1261 aactggccag tgaatgaaca ttatcctaca ggccggcagt taaccatctt caacactcag
     1321 gcgcaaatag ccattggtgg aaaggacaaa ggacgcctct tccaaggcca actctctggg
     1381 ctctattatg atggtttgaa agtactgaac atggcggctg agaacaaccc caatattaaa
     1441 atcaatggaa gtgttcggct ggttggagaa gtcccatcaa ttttgggaac aacacagacg
     1501 acctccatgc caccagaaat gtctactact gtcatggaaa ccactactac aatggcgact
     1561 accacaaccc gtaagaatcg ctctacagcc agcattcagc caacatcaga tgatcttgtt
     1621 tcatctgctg aatgttcaag tgatgatgaa gactttgttg aatgtgagcc gagtacagca
     1681 tacccatacg atgttccaga ttacgcttat ccttacgacg tacctgacta tgctaacccc
     1741 acggagccgg gaatcagacg ggttccgggg gcctcagagg tgatccggga gtcgagcagc
     1801 acaacaggga tggtcgtcgg cattgtggct gctgccgccc tctgcatctt gatcctcctg
     1861 tacgccatgt acaagtacag gaacagggac gaggggtcct atcaagtgga cgagacgcgg
     1921 aactacatca gcaactccgc ccagagcaac ggcacgctca tgaaggagaa gcagcagagc
     1981 tcgaagagcg gccacaagaa acagaaaaac aaggacaggg agtattacgt gtag
//
