LOCUS       sequence-134528-       11337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Small fragment of split HRP fused to the extracellular terminus of 
            NLG1. This is a lentiviral plasmid, but can also be used for 
            transfections. Syanpsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 11337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: Synapsin sHRPb-NLG in FSW lentiviral vector
FEATURES             Location/Qualifiers
     source          1..11337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..3083
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3091..3100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3544..3585
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4047..4056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6029..6617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6082..6102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6500..6511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6620..6636)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6621..6637)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7142..7322
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7348..7365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7354..7578
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7624..8052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7711..7730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7921..7942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8061..8081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8066..8395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8246..8381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8308..8327
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8443..8490
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8509..8883
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9013..9134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9050..9069)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9104..9123
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9183..9199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9183..9199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9196..9218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9207..9223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9231..9237,9238..9255,9256..9261))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9276..9297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9414..9431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9585..10173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9665..9684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10344..11135,11136..11204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10967..10986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11205..11309)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta ataccgccat gcatctgcag agggccctgc gtatgagtgc
     2641 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
     2701 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
     2761 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     2821 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     2881 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     2941 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     3001 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     3061 tcgtgtcgtg cctgagagcg cagtctagac gccaccatgg cacttcccag atgcatgtgg
     3121 ccaaattatg tttggagagc tatgatggca tgtgtggtcc acaggggatc cggtgcccca
     3181 ttgactctct gcttgttggg atgtttgcta cagacttttc acgtactctc tcaaaagacc
     3241 ggtaacttgt ctgcactggt tgactttgac ctgcgcaccc caaccatctt cgacaataag
     3301 tattacgtca atctggagga acagaagggc ctgattcagt ccgaccagga gctgttcagt
     3361 tccccagatg ctaccgacac tattcctctt gtgcgcagct tcgctaatag cacacaaacc
     3421 tttttcaatg ctttcgtgga agctatggat cggatgggaa atataacgcc cctgacgggt
     3481 acgcagggac agataagacg gaactgtagg gtcgtgaaca gcaactctgg ctcgggctcg
     3541 ggcggcaagc ccatccccaa ccccctgctg ggcctggaca gcacccaatt gttggatgat
     3601 gtagacccat tggttactac taactttggc aagattaggg gaattaagaa agaactcaat
     3661 aatgaaattt tgggtcctgt cattcagttt cttggggttc catatgccgc tccaccaaca
     3721 ggagaacatc gtttccagcc tccagaacca ccatctccct ggtctgacat ccggaacgcc
     3781 actcagtttg ctcctgtatg tccccagaat atcattgatg gcagattgcc tgaagttatg
     3841 cttcctgtgt ggttcactaa taacttggat gtggtttcat catacgtcca agaccagagt
     3901 gaagactgtc tatacttaaa catctatgtc ccaactgaag atggtcccct tacaaagaaa
     3961 cacacagatg atttaggtga taatgacggt gctgaagatg aagatattcg ggacagtggg
     4021 ggtcccaaac cagtgatggt gtacatccat ggcggctctt acatggaagg tactggaaat
     4081 ctgtatgatg ggagtgtctt ggcaagctat ggcaatgtga tcgtcatcac agtcaactat
     4141 cggcttgggg tacttggctt cttgagcaca ggggatcagg ctgccaaagg aaactacggg
     4201 ctccttgacc tcatccaggc cctaagatgg accagcgaga acattgggtt ctttggtggt
     4261 gaccccttgc gaatcactgt gtttggatca ggcgctgggg gttcatgtgt caacctgctg
     4321 actttatccc attattctga aggtaaccgt tggagcaatt caaccaaagg actttttcaa
     4381 cgagcaatag ctcagagtgg aacagccctt tccagctggg ctgttagttt ccagcctgca
     4441 aaatacgcta gaattctggc cacaaaagtt ggctgcaatg tttcagatac agtagagtta
     4501 gtagaatgcc tgcagaagaa gccttacaaa gaacttgttg atcaagatgt tcaaccagcc
     4561 cgataccaca tagcctttgg acctgtgatc gatggtgatg taataccaga tgaccctcag
     4621 atactgatgg aacaaggaga gttcctcaac tatgatataa tgttgggagt taaccaaggg
     4681 gaagggttga agtttgtcga aaatatagta gatagtgatg atggtgtatc agccagtgat
     4741 ttcgactttg ctgtttctaa ttttgttgat aatttatatg gatatcctga aggcaaagat
     4801 gttttgagag aaaccattaa attcatgtat actgactggg ctgatcgcca taatcctgaa
     4861 actagaagga agacattgtt ggctttgttt acggaccatc aatgggtagc acctgctgtg
     4921 gccacagcag accttcactc gaactttggc tcacctacat acttctatgc cttttatcat
     4981 cattgccaaa cagatcaagt tccagcttgg gctgatgcag ctcatgggga tgaggttccc
     5041 tatgtgttgg gaatccccat gattggccct acagagttat ttccttgcaa tttctccaag
     5101 aatgatgtga tgttgagtgc agtagtaatg acatactgga cgaattttgc taaaactggt
     5161 gacccaaatc aaccagttcc tcaagacaca aaattcatcc ataccaaacc caaccgcttt
     5221 gaagaagtag catggaccag atattcccag aaagaccagc tttatctcca tattggatta
     5281 aaaccgagag ttaaagagca ttacagagcc aataaggtaa atctctggct ggagctggta
     5341 cctcatctgc ataatctcaa tgacatttct cagtatacct cgacaacaac taaagtgcca
     5401 tccacggaca tcactctcag acctacaagg aagaattcca caccagtcac atcagccttt
     5461 cccactgcca aacaggatga tcccaagcaa caaccaagcc ccttctcggt ggatcagagg
     5521 gactactcca cagagctaag tgtcactatc gcagtggggg cctctctgct gtttctcaac
     5581 atcttggctt ttgcagccct gtactacaag aaggataaga ggagacatga tgtccaccgg
     5641 aggtgcagcc ctcagcgcac gaccaccaac gacctaaccc atgctccaga agaggaaatt
     5701 atgtctctcc aaatgaagca cactgacttg gatcacgagt gtgagtccat ccatccacat
     5761 gaggtggttc ttcggaccgc ctgtccccca gattatactc tagctatgag gaggtcacct
     5821 gatgatattc cactaatgac acctaacacc atcacaatga ttcccaacac tataccaggg
     5881 attcagccct tacatacatt caacacattt actggaggac agaataatac actgccccat
     5941 ccccacccac acccccattc acattcaaca accagggtat aggtcgacgg cgcgcccctg
     6001 cagggaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     6061 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6121 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6181 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6241 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6301 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6361 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6421 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6481 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6541 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6601 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     6661 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6721 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6781 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6841 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6901 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     6961 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     7021 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     7081 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     7141 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7201 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7261 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7321 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7381 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7441 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7501 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7561 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7621 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8041 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8221 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8281 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8341 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8401 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     8461 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     8521 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     8581 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     8641 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     8701 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     8761 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     8821 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8881 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8941 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9001 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9061 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9121 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9241 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9301 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9361 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10261 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10321 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10381 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10441 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10501 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10561 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10621 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10681 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10741 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10801 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10861 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10921 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10981 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11041 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11101 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11161 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
