LOCUS       sequence-134529-        2886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Small fragment of split HRP fused to the extracellular terminus of 
            NLG1. This is a lentiviral plasmid, but can also be used for 
            transfections. Syanpsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: Synapsin sHRPb-NLG in FSW lentiviral vector
FEATURES             Location/Qualifiers
     source          1..2886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             448..489
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      951..960
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcacttc ccagatgcat gtggccaaat tatgtttgga gagctatgat ggcatgtgtg
       61 gtccacaggg gatccggtgc cccattgact ctctgcttgt tgggatgttt gctacagact
      121 tttcacgtac tctctcaaaa gaccggtaac ttgtctgcac tggttgactt tgacctgcgc
      181 accccaacca tcttcgacaa taagtattac gtcaatctgg aggaacagaa gggcctgatt
      241 cagtccgacc aggagctgtt cagttcccca gatgctaccg acactattcc tcttgtgcgc
      301 agcttcgcta atagcacaca aacctttttc aatgctttcg tggaagctat ggatcggatg
      361 ggaaatataa cgcccctgac gggtacgcag ggacagataa gacggaactg tagggtcgtg
      421 aacagcaact ctggctcggg ctcgggcggc aagcccatcc ccaaccccct gctgggcctg
      481 gacagcaccc aattgttgga tgatgtagac ccattggtta ctactaactt tggcaagatt
      541 aggggaatta agaaagaact caataatgaa attttgggtc ctgtcattca gtttcttggg
      601 gttccatatg ccgctccacc aacaggagaa catcgtttcc agcctccaga accaccatct
      661 ccctggtctg acatccggaa cgccactcag tttgctcctg tatgtcccca gaatatcatt
      721 gatggcagat tgcctgaagt tatgcttcct gtgtggttca ctaataactt ggatgtggtt
      781 tcatcatacg tccaagacca gagtgaagac tgtctatact taaacatcta tgtcccaact
      841 gaagatggtc cccttacaaa gaaacacaca gatgatttag gtgataatga cggtgctgaa
      901 gatgaagata ttcgggacag tgggggtccc aaaccagtga tggtgtacat ccatggcggc
      961 tcttacatgg aaggtactgg aaatctgtat gatgggagtg tcttggcaag ctatggcaat
     1021 gtgatcgtca tcacagtcaa ctatcggctt ggggtacttg gcttcttgag cacaggggat
     1081 caggctgcca aaggaaacta cgggctcctt gacctcatcc aggccctaag atggaccagc
     1141 gagaacattg ggttctttgg tggtgacccc ttgcgaatca ctgtgtttgg atcaggcgct
     1201 gggggttcat gtgtcaacct gctgacttta tcccattatt ctgaaggtaa ccgttggagc
     1261 aattcaacca aaggactttt tcaacgagca atagctcaga gtggaacagc cctttccagc
     1321 tgggctgtta gtttccagcc tgcaaaatac gctagaattc tggccacaaa agttggctgc
     1381 aatgtttcag atacagtaga gttagtagaa tgcctgcaga agaagcctta caaagaactt
     1441 gttgatcaag atgttcaacc agcccgatac cacatagcct ttggacctgt gatcgatggt
     1501 gatgtaatac cagatgaccc tcagatactg atggaacaag gagagttcct caactatgat
     1561 ataatgttgg gagttaacca aggggaaggg ttgaagtttg tcgaaaatat agtagatagt
     1621 gatgatggtg tatcagccag tgatttcgac tttgctgttt ctaattttgt tgataattta
     1681 tatggatatc ctgaaggcaa agatgttttg agagaaacca ttaaattcat gtatactgac
     1741 tgggctgatc gccataatcc tgaaactaga aggaagacat tgttggcttt gtttacggac
     1801 catcaatggg tagcacctgc tgtggccaca gcagaccttc actcgaactt tggctcacct
     1861 acatacttct atgcctttta tcatcattgc caaacagatc aagttccagc ttgggctgat
     1921 gcagctcatg gggatgaggt tccctatgtg ttgggaatcc ccatgattgg ccctacagag
     1981 ttatttcctt gcaatttctc caagaatgat gtgatgttga gtgcagtagt aatgacatac
     2041 tggacgaatt ttgctaaaac tggtgaccca aatcaaccag ttcctcaaga cacaaaattc
     2101 atccatacca aacccaaccg ctttgaagaa gtagcatgga ccagatattc ccagaaagac
     2161 cagctttatc tccatattgg attaaaaccg agagttaaag agcattacag agccaataag
     2221 gtaaatctct ggctggagct ggtacctcat ctgcataatc tcaatgacat ttctcagtat
     2281 acctcgacaa caactaaagt gccatccacg gacatcactc tcagacctac aaggaagaat
     2341 tccacaccag tcacatcagc ctttcccact gccaaacagg atgatcccaa gcaacaacca
     2401 agccccttct cggtggatca gagggactac tccacagagc taagtgtcac tatcgcagtg
     2461 ggggcctctc tgctgtttct caacatcttg gcttttgcag ccctgtacta caagaaggat
     2521 aagaggagac atgatgtcca ccggaggtgc agccctcagc gcacgaccac caacgaccta
     2581 acccatgctc cagaagagga aattatgtct ctccaaatga agcacactga cttggatcac
     2641 gagtgtgagt ccatccatcc acatgaggtg gttcttcgga ccgcctgtcc cccagattat
     2701 actctagcta tgaggaggtc acctgatgat attccactaa tgacacctaa caccatcaca
     2761 atgattccca acactatacc agggattcag cccttacata cattcaacac atttactgga
     2821 ggacagaata atacactgcc ccatccccac ccacaccccc attcacattc aacaaccagg
     2881 gtatag
//
