LOCUS       sequence-134531-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Large fragment of split HRP targeted for expression in the lumen of 
            the endoplasmic reticulum (ER). CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pDisplay sHRPa-KDEL
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacggggccc agccggccag atctgattat aaggacgatg atgacaaaca gcttactcca
      121 actttctacg ataactcttg tcctaacgtg agcaacatcg tgcgagatat tatcgtgaac
      181 gagttgcggt ctgatccgag gatagccgct tcaattttgc ggctgcattt ccacgattgt
      241 tttgtaaacg gctgtgatgc ttccatcctc ctcgacaata ccacctcttt caggactgaa
      301 aaggatgcct tcggtaacgc gaatagtgct agagggtttt ccgtgatcga tagaatgaaa
      361 gccgctgtgg agagcgcctg ccccgggaca gttagttgtg cagaccttct gaccatagcg
      421 gcccagcaat ccgtgacgct ggccggtggc cccagctgga gggtccccct gggaaggagg
      481 gactctctgc aggcttttct ggacttggca aacgcaaacc tgcctgctcc cttcttcact
      541 cttccccagc tgaaggattc cttcaggaat gtcggcctga accgaagtag tgatttggtg
      601 gctctgtccg gaggccacac attcggcaag agtcagtgca ggttcatcat ggacagactg
      661 tataactttt ccaacaccgg cttgcctgac cctaccctga acacaacata cttgcagact
      721 ctcagaggac tgtgtccgct caatggcggc agcggcaagg acgagctgta a
//
