LOCUS       sequence-134537-         435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Small fragment of split HRP targeted for expression in the lumen of 
            the endoplasmic reticulum (ER). CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pDisplay sHRPb-KDEL
FEATURES             Location/Qualifiers
     source          1..435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             85..126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacggggccc agccggccag atctggcaag cccatcccca accccctgct gggcctggac
      121 agcaccaact tgtctgcact ggttgacttt gacctgcgca ccccaaccat cttcgacaat
      181 aagtattacg tcaatctgga ggaacagaag ggcctgattc agtccgacca ggagctgttc
      241 agttccccag atgctaccga cactattcct cttgtgcgca gcttcgctaa tagcacacaa
      301 acctttttca atgctttcgt ggaagctatg gatcggatgg gaaatataac gcccctgacg
      361 ggtacgcagg gacagataag acggaactgt agggtcgtga acagcaactc tggcagcggc
      421 aaggacgagc tgtaa
//
