LOCUS       sequence-134539-        2925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Large fragment of split HRP fused to the N-terminus of the yeast 
            mating protein Aga1P. When linearized, this vector incorporates into
            the yeast genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: YIP sHRPa-Aga1P
FEATURES             Location/Qualifiers
     source          1..2925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             751..792
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgacattat ctttcgctca ttttacctac ctgttcacaa tattgttggg attaactaat
       61 attgccttgg catctgatcc agaaacggga ggttctctta agcagcttac tccaactttc
      121 tacgataact cttgtcctaa cgtgagcaac atcgtgcgag atattatcgt gaacgagttg
      181 cggtctgatc cgaggatagc cgcttcaatt ttgcggctgc atttccacga ttgttttgta
      241 aacggctgtg atgcttccat cctcctcgac aataccacct ctttcaggac tgaaaaggat
      301 gccttcggta acgcgaatag tgctagaggg ttttccgtga tcgatagaat gaaagccgct
      361 gtggagagcg cctgccccgg gacagttagt tgtgcagacc ttctgaccat agcggcccag
      421 caatccgtga cgctggccgg tggccccagc tggagggtcc ccctgggaag gagggactct
      481 ctgcaggctt ttctggactt ggcaaacgca aacctgcctg ctcccttctt cactcttccc
      541 cagctgaagg attccttcag gaatgtcggc ctgaaccgaa gtagtgattt ggtggctctg
      601 tccggaggcc acacattcgg caagagtcag tgcaggttca tcatggacag actgtataac
      661 ttttccaaca ccggcttgcc tgaccctacc ctgaacacaa catacttgca gactctcaga
      721 ggactgtgtc cgctcaatgg cggctcgggc ggcaagccca tccccaaccc cctgctgggc
      781 ctggacagca ccggtagcgg aggcggaggg tcgagcggcg ctagcggtgg ttctattcta
      841 gtgacgataa ccaagacaaa cgatgcaaat ggggttgtta caactacagt ttcacccgcg
      901 ctagtctcca catccactat cgttcaagct ggcactacga cattgtatac gacttggtgt
      961 ccattgacgg tatccacttc atctgctgcc gaaataagtc cttcaatatc gtacgctact
     1021 accctatcca gatttagtac tttgacatta tctacagaag tctgctccca tgaggcatgt
     1081 ccttcgtcat cgacgttgcc aaccaccacc ttatctgtga cttccaagtt cacttcatat
     1141 atttgcccta cttgtcacac aaccgctatc agctcattat ccgaagtagg aactacaacc
     1201 gtggtatcat ccagcgccat tgaaccatca agtgcctcta taatctcacc tgtcacctct
     1261 acactttcga gtacaacatc gtccaatcca actactacct ccctaagttc gacatctaca
     1321 tctccaagct ctacatctac atctccaagc tctacatcta cctcatcaag ttcgacatct
     1381 acctcatcaa gttcgacatc tacctcatca agttcgacat ctacatctcc aagttcgaca
     1441 tccacatctt caagtttgac atccacatct tcaagttcta catctacatc ccaaagttct
     1501 acatctacct catcaagttc gacatctaca tctccaagct ctacatctac ctcatcaagt
     1561 tcaacatcta catctccaag ttctaaatct acttctgcaa gctccacttc cacttcttca
     1621 tattcaacat ctacatcccc aagtttgact tcttcatctc caactttggc ttccacttct
     1681 ccaagttcaa catctattag ctctactttt actgattcaa cttcatccct tggctcctct
     1741 atagcatctt catcaacgtc tgtgtcatta tacagcccat ccacacctgt ttactccgtc
     1801 ccttcgactt cgtcaaatgt tgcaactcct tctatgactt cttcaactgt tgaaacaact
     1861 gttagttcac aaagttcgtc tgaatatatc accaaatcct caatttctac tactatccca
     1921 tcattttcca tgtctacata tttcaccact gttagtggag tcactacaat gtatacgaca
     1981 tggtgtcctt atagctctga atctgagact agcacattaa ccagtatgca tgaaacggtt
     2041 acaacagacg ctacagtctg cactcacgag tcttgcatgc cctcgcagac aacaagtttg
     2101 attacatctt ctataaaaat gtccactaaa aacgtcgcaa cttctgtaag cacctcaacg
     2161 gttgaatcct catatgcatg ctccacatgt gctgaaacgt cacactcgta ttcttccgtg
     2221 caaacagctt catcaagttc tgtaacacag cagaccacat ccacaaagag ttgggtaagt
     2281 tcaatgacaa cttcggatga agatttcaat aagcacgcta ccggtaagta tcatgtaaca
     2341 tcttcaggta cctcaaccat ttcgactagt gtaagtgaag ccacgagtac atcaagcatt
     2401 gactcagaat ctcaagaaca atcatcacac ttattatcga catcggtcct ttcatcctcc
     2461 tccttgtctg ctacattatc ctctgacagt actattttgc tattcagttc tgtatcatca
     2521 ctaagtgtcg aacagtcacc agttaccaca cttcaaattt cttcaacatc agagatttta
     2581 caacccactt cttccacagc tattgctaca atatctgcct ctacatcatc actttccgca
     2641 acatctatct ctacaccatc tacctctgtg gaatcgacta ttgaatcttc atcattgact
     2701 ccgacggtat cttctatttt cctctcatca tcatctgctc cctcttctct acaaacatct
     2761 gttaccacta cagaagtttc cactacttca atctccatac aataccaaac ttcatcaatg
     2821 gtaacaatta gccaatatat gggcagtgga tcgcaaacgc gtttgccatt aggaaagttg
     2881 gtcttcgcca tcatggcagt tgcttgcaat gtaattttca gttaa
//
