LOCUS       sequence-134541-        1380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full-length horseradish peroxidase (HRP) fused to the C-terminus of 
            the yeast mating protein Aga2P. Galactose-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pCTCON2 Aga2P-HRP
FEATURES             Location/Qualifiers
     source          1..1380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             280..291
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             292..318
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             325..345
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1348..1377
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgcagttac ttcgctgttt ttcaatattt tctgttattg cttcagtttt agcacaggaa
       61 ctgacaacta tatgcgagca aatcccctca ccaactttag aatcgacgcc gtactctttg
      121 tcaacgacta ctattttggc caacgggaag gcaatgcaag gagtttttga atattacaaa
      181 tcagtaacgt ttgtcagtaa ttgcggttct cacccctcaa caactagcaa aggcagcccc
      241 ataaacacac agtatgtttt taaggacaat agctcgacga ttgaaggtag atacccatac
      301 gacgttccag actacgctct gcaggagaac ttgtactttc aaggtctgca ggctagtggt
      361 ggaggaggct ctggtggagg cggtagcgga ggcggagggt cggctagcca gcttactcca
      421 actttctacg ataactcttg tcctaacgtg agcaacatcg tgcgagatac tatcgtgaac
      481 gagttgcggt ctgatccgag gatagccgct tcaattttgc ggctgcattt ccacgattgt
      541 tttgtaaacg gctgtgatgc ttccatcctc ctcgacaata ccacctcttt caggactgaa
      601 aaggatgcct tcggtaacgc gaatagtgct agagggtttc ccgtgatcga tagaatgaaa
      661 gccgctgtgg agagcgcctg cccccggaca gttagttgtg cagaccttct gaccatagcg
      721 gcccagcaat ccgtgacgct ggccggtggc cccagctgga gggtccccct gggaaggagg
      781 gactctctgc aggcttttct ggacttggca aacgcaaacc tgcctgctcc cttcttcacc
      841 cttccccagc tgaaggattc cttcaggaat gtcggcctga accgaagtag tgatttggtg
      901 gctctgtccg gaggccacac attcggcaag aatcagtgca ggttcatcat ggacagactg
      961 tataactttt ccaacaccgg cttgcctgac cctaccctga acacaacata cttgcagact
     1021 ctcagagggc tgtgtccgct caatggcaac ttgtctgcac tggttgactt tgacctgcgc
     1081 accccaacca tcttcgacaa taagtattac gtcaatctgg aggaacagaa gggcctgatt
     1141 cagtccgacc aggagctgtt cagttcccca aatgctaccg acactattcc tcttgtgcgc
     1201 agcttcgcta atagcacaca aacctttttc aatgctttcg tggaagctat ggatcggatg
     1261 ggaaatataa cgcccctgac gggtacgcag ggacagataa gactgaactg tagggtcgtg
     1321 aacagcaact ctggctcggg cggatccgaa caaaagctta tttctgaaga ggacttgtaa
//
