LOCUS       sequence-134542-        1264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes a toxin gene (relE) under the control
            of rhamnose induceable promoter (PrhaB) to be used as a negative 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-217
FEATURES             Location/Qualifiers
     source          1..1264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      86..172
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      264..291
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     regulatory      345..354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        494..612
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      983..1229
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 ccgcggccca aagtgccacc tgcatcgatg gcccccgatg gtagtgtggg gtctccccat
       61 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      121 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
      181 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
      241 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtggcc
      301 agtgccaagc ttgcatgcct gcagctgtcc ggaactagtc gaggccatgg tggcctcctg
      361 atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac
      421 ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg
      481 gtgagcatca catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct
      541 ggttgccaat ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt
      601 agactggtcg taatgaaatt cagcaggatc accatatggc gtattttctg gattttgacg
      661 agcgggcact aaaggaatgg cgaaagctgg gctcgacggt acgtgaacag ttgaaaaaga
      721 agctggttga agtacttgag tcaccccgga ttgaagcaaa caagctccgt ggtatgcctg
      781 attgttacaa gattaagctc cggtcttcag gctatcgcct tgtataccag gttatagacg
      841 agaaagttgt cgttttcgtg atttctgttg ggaaaagaga acgctcggaa gtatatagcg
      901 aggcggtcaa acgcattctc tgaggatccg cgaattctca tgtttgacag cttatcactg
      961 atcagtgaat taatggcgat gacgcatcct cacgataata tccgggtagg cgcaatcact
     1021 ttcgtctcta ctccgttaca aagcgaggct gggtatttcc cggcctttct gttatccgaa
     1081 atccactgaa agcacagcgg ctggctgagg agataaataa taaacgaggg gctgtatgca
     1141 caaagcatct tctgttgagt taagaacgag tatcgagatg gcacatagcc ttgctcaaat
     1201 tggaatcagg tttgtgccaa taccagtaga aacagacgaa gaagctagct ttgcactgga
     1261 tggg
//
