LOCUS       sequence-134544-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  multi-cistronic Vector for expression for Fly_FUCCI probes in insect
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Zielke N, Korzelius J, van Straaten M, Bender K, Schuhknecht GF, 
            Dutta D, Xiang J, Edgar BA
  TITLE     Fly-FUCCI: A versatile tool for studying cell proliferation in 
            complex tissues.
  JOURNAL   Cell Rep. 2014 Apr 24;7(2):588-98. doi: 
            10.1016/j.celrep.2014.03.020. Epub 2014 Apr 13.
  PUBMED    24726363
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.020"; journalName: "Cell Rep"; date: 
            "2014-04-24- 24"; volume: "7"; issue: "2"; pages: "588-98"
COMMENT     Sequence Label: Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230...
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..681
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             7..681
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(137..155)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     405..424
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     599..622
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             718..738
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1543..1596
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1615..1617,1618..1620,1621..2331)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1615..1617,1618..1620,1621..2331)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1660..1681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1921..1940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2268..2289
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3064..3117
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3124..3927
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3187..3206)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3797..3816
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3935..4069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3959..3978)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4013..4032
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4691..5279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4771..4790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5450..6241,6242..6310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6073..6092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6311..6415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6483..6501
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6565..9077
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 ggtaccatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg
       61 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      121 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
      181 gacatcctgt cccctcagtt ccagtacggc tccaaggcct acgtgaagca ccccgccgac
      241 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      301 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      361 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
      421 aagaccatgg gctgggaggc ctccaccgag cggatgtacc ccgaggacgg cgccctgaag
      481 ggcgagatca agatgaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag
      541 accacctaca tggccaagaa gcccgtgcag ctgcccggcg cctacaagac cgacatcaag
      601 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag
      661 ggccgccact ccaccggcgc ccaccggtat acaggctccg cggcggcccc cttcacccca
      721 aaaaagaaac gtaaggttat ggtgggcaca acactgaaaa tgcgtggcga tgagaacgct
      781 tcggagaact tcaagcaagt gcaattgaag aaattgacgg ttccttccat ggaggcaaca
      841 acaaaacgcg cggccttggg cgatttgcag aatcgcggca taagtcgtcc catcgcagcg
      901 aaggatgcgg cacagaaaga ctccaaggat ctcaagctca cagacgccct gcgcaatgcc
      961 aaagctcggg tggacagcca ctggaagaaa cagccactgg gcagcaccaa tggcaatggc
     1021 aatggcgccg ttccgcccaa ggtcaacgag gggggcgtgt cggcgttttt gcgttcgaat
     1081 tcggtgcgca atcgcgttcc gaccaagacc actgtagaac ccactaaagt tacagtcaag
     1141 tccagttctt ccgagaacgt gaacgagccc accttaaagc gcgaggacag caatctgtcg
     1201 aagaagtcgc tgaccaaact gcgtgccgct ttggccaaac ccgtgatggg agtttcagga
     1261 attcgacggg aaccagtagc tgtttcccgc aaagaggcag agaccaagaa ggaactgcca
     1321 gaaaccaaga aggactcact ggaagtgaaa aaggatgcga ccaggatgcc ccttattagg
     1381 ggcaacagtg cagtcactac gaccacatct acgatgccca ccaccatgtc cctttccagc
     1441 aagcgcttgg ctggaatcga ggacattgat gccaatgaca aggagaacct ggtactggtc
     1501 tccgaatatg taaacgacat ctacgactac ttgcgcggcc gcgaaggacg cggcagccta
     1561 ctgacttgcg gagatgtcga agagaaccct ggccctggtt ccgatatctc tagaatggtg
     1621 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1681 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1741 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1801 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1861 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1921 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1981 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2041 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2101 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2161 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2221 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2281 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcaccggtat
     2341 acaggctccg cggccgcccc cttcaccatg tccaagttct ttgtgaatgt tgcccccatc
     2401 aacaacagca acagcagcag tagccacaca accaccagca gcaacaccca gcgccaccag
     2461 cagcaccaac aacactatgg cgggagcggg acaaccggcc acacgatggt ggcccgcaga
     2521 ctcaactacg atctgcacgg cggcaccacc agcatcaata acaacaacaa cattgtgatc
     2581 aaaaacgagt ccgtcgactt ggactacgat cacgtcctga gcagcagcga cagcaacagc
     2641 aacggcggcg tggcagccca cctgcgggat catgtctaca tcagtctgga caaggggcac
     2701 aacacggggg cagtggcaac agcagcggcg gcggcaacgg cgggccaaac gcagcagcaa
     2761 ctgcagcagc agcaccacca ccaaaatcag cagcaacgca aggcgaccgg caaatccaac
     2821 gatatcacaa attattacaa ggtcaaacgt cggccacacg ccgtcagcga tgagattcac
     2881 ccgaagaagc aggccaagca gagcgcccac caccagacgg tgtatcagaa gcatacggct
     2941 agcagtgcgc cccagcagct ccggcacagc caccaccagc tgcgccacga cgcggatgcc
     3001 gaactggacg aggatgtggt ggagcgggtg gccaagccgg catcgcatca cccctttaag
     3061 cttgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctgct
     3121 agcatgggat cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     3181 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     3241 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3301 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     3361 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     3421 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     3481 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     3541 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     3601 gatctggacg aagaacatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     3661 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     3721 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     3781 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagaacttgg cggcgaatgg
     3841 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     3901 tatcgccttc ttgacgagtt cttctgactc gagggatcca gacatgataa gatacattga
     3961 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4021 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     4081 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     4141 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct
     4201 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     4261 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     4321 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     4381 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     4441 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     4501 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     4561 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     4621 aaggatcgct cgcggctctt accagccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4861 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     4921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5101 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     5161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5341 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5401 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5461 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5521 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5581 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5641 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5701 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5761 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5821 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5881 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5941 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6001 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6061 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6121 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6181 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6241 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6301 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6361 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6421 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     6481 aaaataggcg tatcacgagg ccctttcgtc ttcaaggaat taattctata ttctaaaaac
     6541 acaaatgata cttctaaaaa aaatcatgaa tggcatcaac tctgaatcaa atctttgcag
     6601 atgcacctac ttctcatttc cactgtcaca tcatttttcc agatctcgct gcctgttatg
     6661 tggcccacaa accaagacac gttttatggc cattaaagct ggctgatcgt cgccaaacac
     6721 caaatacata tcaatatgta cattcgagaa agaagcgatc aaagaagcgt cttcgggcga
     6781 gtaggagaat gcggaggaga aggagaacga gctgatctag tatctctcca caatccaatg
     6841 ccaactgacc aactggccat attcggagca atttgaagcc aatttccatc gcctggcgat
     6901 cgctccattc ttggctatat gtttttcacc gttcccgggg ccattttcaa agactcgtcg
     6961 gtaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt
     7021 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc
     7081 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt
     7141 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt
     7201 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaca ggctgatttc
     7261 tgcgtctttt gccgaacgca gtgcatgtgc ggttgttgtt ttttggaata gtttcaattt
     7321 tcggactgtc cgctttgatt tcagtttctt ggcttattca aaaagcaaag taaagccaaa
     7381 aaagcgagat ggcaatacca aatgcggcaa aacggtagtg gaaggaaagg ggtgcggggc
     7441 agcggaagga agggtggggc ggggcgtggc ggggtctgtg gctgggcgcg acgtcaccga
     7501 cgttggagcc actcctttga ccatgtgtgc gtgtgtgtat tattcgtgtc tcgccactcg
     7561 ccggttgttt ttttcttttt atctcgctct ctctagcgcc atctcgtacg catgctcaac
     7621 gcaccgcatg ttgccgtgtc ctttatgcgt cattttggct cgaaataggc aattatttaa
     7681 acaaagatta gtcaacgaaa acgctaaaat aaataagtct acaatatggt tacttattgc
     7741 catgtgtgtg cagccaacga tagcaacaaa agcaacaaca cagtggcttt ccctctttca
     7801 ctttttgttt gcaagcgcgt gcgagcaaga cggcacgacc ggcaaacgca attacgctga
     7861 caaagagcag acgaagtttt ggccgaaaaa catcaaggcg cctgatacga atgcatttgc
     7921 aataacaatt gcgatattta atattgttta tgaagctgtt tgacttcaaa acacacaaaa
     7981 aaaaaaataa aacaaattat ttgaaagaga attaggaatc ggacagctta tcgttacggg
     8041 ctaacagcac accgagacga aatagcttac ctgacgtcac agcctctgga agaactgccg
     8101 ccaagcagag agagagagaa aaagagggag agcagcttag accgcatgtg cttgtgtgtg
     8161 aggcgtctct ctcttcgtct cctgtttgcg caaacgcata gactgcactg agaaaatcga
     8221 ttacctattt tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca
     8281 atcacattca atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga
     8341 gcaaaaatgc tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt
     8401 tctctcgtct ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt
     8461 tctacgacta gtgatgcccc tactagaaga tgtgtgttgc acaaatgtcc ctgaataacc
     8521 aatttgaagt gcagatagca gtaaacgtaa gctaatatga atattattta actgtaatgt
     8581 tttaatatcg ctggacatta ctaataaacc cactataaac acatgtacat atgtatgttt
     8641 tggcatacaa tgagtagttg gggaaaaaat gtgtaaaagc accgtgacca tcacagcata
     8701 aagataacca gctgaagtat cgaatatgag taacccccaa attgaatcac atgccgcaac
     8761 tgataggacc catggaagta cactcttcat ggcgatatac aagacacaca caagcacgaa
     8821 cacccagttg cggaggaaat tctccgtaaa tgaaaaccca atcggcgaac aattcatacc
     8881 catatatggt aaaagttttg aacgcgactt gagagcggag agcattgcgg ctgataaggt
     8941 tttagcgcta agcgggcttt ataaaacggg ctgcgggacc agttttcata tcactaccgt
     9001 ttgagttctt gtgctgtgtg gatactcctc ccgacacaaa gccgctccat cagccagcag
     9061 tcgtctaatc cagagacccc ggatcgg
//
