LOCUS       sequence-134548-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP when FLP is expressed by another vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Fon-copGFP
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..774)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     regulatory      795..804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(809..841)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 agttcctatt ctctgaaagt ataggaactt cggcgcgcct tagcgagatc cggtggagcc
       61 gggtccggcg gtgccgtcca cggcagaatt ggacgactga gcgcgggatc tggcgaaggc
      121 aatgggggtc ttgaaggcgt gctggtactc cacgatgccc agctcggtgt tgctgtgcag
      181 ctcctccacg cggcggaagg cgaacatggg gcccccgttc tgcaggatgc tggggtggat
      241 ggcgctcttg aagtgcatgt ggctgtccac cacgaagctg tagtagccgc cgtcgcgcag
      301 gctgaaggtg cgggcgaagc tgcccaccag cacgttatcg cccatggggt gcaggtgctc
      361 cacggtggcg ttgctgcgga tgatcttgtc ggtgaagatc acgctgtcct cggggaagcc
      421 ggtgcccacc accttgaagt cgccgatcac gcggccggcc tcgtagcggt agctgaagct
      481 cacgtgcagc acgccgccgt cctcgtactt ctcgatgcgg gtgttggtgt agccgccgtt
      541 gttgatggcg tgcaggaagg ggttctcgta gccgctgggg taggtgccga agtggtagaa
      601 gccgtagccc atcacgtggc tcagcaggta ggggctgaag gtcagggcgc ctttggtgct
      661 cttcatcttg ttggtcatgc ggccctgctt gggggtgccc tctccgccgc ccaccagctc
      721 gaactccacg ccgttcaggg tgccggtgat gcggcactcg atctccatgg cgggcaggcc
      781 gctctcgtcg ctctccatgg tggctagcga agttcctata ctttcagaga ataggaactt
      841 cga
//
