LOCUS       sequence-134551-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express tdTomato when FLP is expressed by another vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Fon-tdTomato
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..60
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(209..227)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     477..496
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     671..694
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcgaa gttcctattc tctgaaagta taggaacttc
       61 gctagccccg ggccaatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc
      121 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
      181 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      241 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
      301 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
      361 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
      421 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
      481 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
      541 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
      601 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
      661 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      721 gagcgctccg agggccgcca ccacctgttc ctggggcatg gcatcggcag caccggcagc
      781 ggcagctccg gcaccg
//
