LOCUS       sequence-134554-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Glover CP, Heywood DJ, Bienemann AS, Deuschle U, Kew JN, Uney JB
  TITLE     Adenoviral expression of CREB protects neurons from apoptotic and 
            excitotoxic stress.
  JOURNAL   Neuroreport. 2004 May 19;15(7):1171-5.
  PUBMED    15129168
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroreport."; date: "2004-05-19"; volume: "15(7)"; pages: "1171-5"
COMMENT     Sequence Label: pXCX.CMV.ACREB.WPRE
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..786)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(113..133)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     728..756
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     regulatory      783..792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ggatcctcta gatgcatgct cgagcggccg
       61 ccctcgagtt tatttgtata gttcatccat gccatgtgta atcccagcag ctgttacaaa
      121 ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt
      181 ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg
      241 ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt tgaagttaac
      301 tttgattcca ttcttttgtt tgtctgccat gatgtataca ttgtgtgagt tatagttgta
      361 ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt ttaactcgat
      421 tctattaaca agggtatcac cttcaaactt gacttcagca cgtgtcttgt agttcccgtc
      481 atctttgaaa aatatagttc tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa
      541 gtcatgccgt ttcatatgat ctgggtatct cgcaaagcat tgaagaccat acgcaaaagt
      601 agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa atttaagggt
      661 aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt tgtgcccatt
      721 aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt
      781 acccatggtg gcggcccggt cgactctagc tagaggtacc acgcgtgcca tattatcatc
      841 gtgtttttca a
//
