LOCUS       sequence-134555-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP and puromycin resistance gene under EF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..699
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             784..837
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(838..857)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 attggatcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
       61 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
      121 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
      181 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
      241 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
      301 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
      361 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
      421 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
      481 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
      541 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
      601 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
      661 gagtaccagc acgccttcaa gacccccatt gccttcgcca gatcccgcgc tcagtcgtcc
      721 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcaa gcttggcagt
      781 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg
      841 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccc
//
