LOCUS       sequence-134561-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mCherry and puromycin resistance gene under CMV promoter. 
            3rd gen lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CMV-mCherry-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..782
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(220..238)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     488..507
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             798..851
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agattctaga
       61 gctagggatc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
      121 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      181 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      241 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      301 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      361 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      421 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      481 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      541 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      601 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      661 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      721 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      781 agaagcttgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga
      841 atcctggccc aatgaccgag tacaagcc
//
