LOCUS       sequence-134564-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gene of interest under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CMV
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(108..132)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(129..332)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(158..178)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    422..538
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(805..849)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcgcg gccgccccgg ggctagcgga tccctagctc
       61 tagaatcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc
      121 actaaacgag ctctgcttat ataaacctcc caccgtacac gcctaccgcc catttgcgtc
      181 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
      241 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
      301 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
      361 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataata ctagtatcga
      421 taaaattttg aattttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
      481 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaagt
      541 taaccgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
      601 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
      661 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
      721 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
      781 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
      841 ttcattcttt tcttgctggt tttgcgattc ttcaattaag
//
