LOCUS       sequence-134566-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gene of interest under EF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          61..264
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(84..104)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcgcg gccgcccggg gatccaattc ctcacgacac
       61 ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc
      121 aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc
      181 agtctccacc cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa
      241 agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc
      301 cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga
      361 ctcacccgcc cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc
      421 gggaagcggc catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca
      481 gggcggggcg atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga
      541 ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg
      601 ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag
      661 atcttggccc gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca
      721 aaaattttaa atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca
      781 gattcgcacg cggcggccc
//
