LOCUS       sequence-134571-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gene of interest under PGK (phosphoglycerate kinase I) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-PGK
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..533
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(69..92)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     466..485
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(629..649)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aaattttatc gatagatacg aattaattct accgggtagg ggaggcgctt ttcccaaggc
       61 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
      121 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
      181 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
      241 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
      301 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
      361 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
      421 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
      481 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacggatc
      541 cgctagcgtt taaacgcggc cgcgaattcg tcgacaatca acctctggat tacaaaattt
      601 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
      661 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
      721 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
      781 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
      841 agctcctttc cgggactttc gctttccc
//
