LOCUS       sequence-134572-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gene of interest under PGK (phosphoglycerate kinase I) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-PGK
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(58..557)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(106..125)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     499..522
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    584..700
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcgcg gccgcgttta aacgctagcg gatccgtcga
       61 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
      121 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
      181 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
      241 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
      301 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
      361 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
      421 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
      481 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
      541 aagcgcctcc cctacccggt agaattaatt cgtatctatc gataaaattt tgaattttgt
      601 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
      661 ccctgcactg taccccccaa tccccccttt tcttttaaaa gttaaccgat accgtcgaga
      721 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
      781 ttcgggcctg tcgggtcccc tcgg
//
