LOCUS       sequence-134577-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Luc2 and copGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     express gene of interest under truncated EF-1 promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Luc2-P2A-copGFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..770)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
ORIGIN
        1 atccagaggt tgattgtcga cgaattcggc gcgccttagc gagatccggt ggagccgggt
       61 ccggcggtgc cgtccacggc agaattggac gactgagcgc gggatctggc gaaggcaatg
      121 ggggtcttga aggcgtgctg gtactccacg atgcccagct cggtgttgct gtgcagctcc
      181 tccacgcggc ggaaggcgaa catggggccc ccgttctgca ggatgctggg gtggatggcg
      241 ctcttgaagt gcatgtggct gtccaccacg aagctgtagt agccgccgtc gcgcaggctg
      301 aaggtgcggg cgaagctgcc caccagcacg ttatcgccca tggggtgcag gtgctccacg
      361 gtggcgttgc tgcggatgat cttgtcggtg aagatcacgc tgtcctcggg gaagccggtg
      421 cccaccacct tgaagtcgcc gatcacgcgg ccggcctcgt agcggtagct gaagctcacg
      481 tgcagcacgc cgccgtcctc gtacttctcg atgcgggtgt tggtgtagcc gccgttgttg
      541 atggcgtgca ggaaggggtt ctcgtagccg ctggggtagg tgccgaagtg gtagaagccg
      601 tagcccatca cgtggctcag caggtagggg ctgaaggtca gggcgccttt ggtgctcttc
      661 atcttgttgg tcatgcggcc ctgcttgggg gtgccctctc cgccgcccac cagctcgaac
      721 tccacgccgt tcagggtgcc ggtgatgcgg cactcgatct ccatggcggg caggccgctc
      781 tcgtcgctct ccataggtcc agggttctcc tccacgtctc
//
