LOCUS       sequence-134579-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Luc2 and tdTomato under EF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     express gene of interest under truncated EF-1 promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Luc2-P2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(333..352)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     602..620
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tctttcacaa attttgtaat ccagaggttg attgtcgacg aattcttatt acttgtacag
       61 ctcgtccatg ccgtacagga acaggtggtg gcggccctcg gagcgctcgt actgttccac
      121 gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gtgtccacgt agtagtagcc
      181 gggcagttgc acgggcttct tggccatgta gatggtcttg aactccacca ggtagtggcc
      241 gccgtccttc agcttcaggg cctggtggat ctcgcccttc agcacgccgt cgcgggggta
      301 caggcgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcggg
      361 ggggaagttg gtgccgcgca tcttcacctt gtagatcagc gtgccgtcct gcagggagga
      421 gtcctgggtc acggtcacca gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa
      481 gccctcgggg aaggacagct tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc
      541 cttggagccg tacatgaact ggggggacag gatgtcccag gcgaagggca gggggccgcc
      601 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
      661 ctcgatctcg aactcgtggc cgttcatgga gccctccatg cgcaccttga agcgcatgaa
      721 ctctttgatg acggccatgt tgttgtcctc ggaggaggcg gtgccggagc tgccgctgcc
      781 ggtgctgccg atgccatgcc ccaggaacag gtggtggcgg ccctcggagc gctcgtactg
      841 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta
      901 gtagccgggc agttgcacgg gcttcttggc catgtagatg gtcttgaact
//
