LOCUS       sequence-134582-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express shRNA against mouse TNF-alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ogawa N, Kawai H, Terashima T, Kojima H, Oka K, Chan L, Maegawa H
  TITLE     Gene therapy for neuropathic pain by silencing of TNF-alpha 
            expression with lentiviral vectors targeting the dorsal root 
            ganglion in mice.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92073. doi: 
            10.1371/journal.pone.0092073. eCollection 2014.
  PUBMED    24642694
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92073. doi"; pages: " 
            10.1371/journal.pone.0092073. eCollection 2014"
COMMENT     Sequence Label: pLL3.7-TNFa-shRNA3
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..83
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     68..84
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(126..159)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        275..578
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        579..782
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     733..753
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      821..830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agccttgttt gcctccctct catcagttct atctcgagat agaactgatg agagggaggt
       61 tttttctcga ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg
      121 acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag
      181 cggccgcgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
      241 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      301 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      361 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      421 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      481 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      541 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      601 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      661 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      721 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      781 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagca
//
