LOCUS       sequence-134583-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express shRNA against mouse TNF-alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Ogawa N, Kawai H, Terashima T, Kojima H, Oka K, Chan L, Maegawa H
  TITLE     Gene therapy for neuropathic pain by silencing of TNF-alpha 
            expression with lentiviral vectors targeting the dorsal root 
            ganglion in mice.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92073. doi: 
            10.1371/journal.pone.0092073. eCollection 2014.
  PUBMED    24642694
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92073. doi"; pages: " 
            10.1371/journal.pone.0092073. eCollection 2014"
COMMENT     Sequence Label: pLL3.7-TNFa-shRNA3
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(4..207)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(33..53)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        208..511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    627..660
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(702..718)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(703..719)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
       61 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
      121 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
      181 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
      241 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
      301 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
      361 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
      421 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
      481 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
      541 tgaccccgta attgattact attaataact aatgcatggc ggtaatacgg ttatccacgc
      601 ggccgcttaa gcttggaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
      661 taggtccctc gacctgctgg aatctcgtga agcgagctta tcgataccgt cgacctcgag
      721 aaaaaacctc cctctcatca gttctatctc gagatagaac tgatgagagg gaggcaaaca
      781 aggcttttct ccaagggata tttatagtct caa
//
