LOCUS       sequence-134589-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRaspberry-ER-3
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..756
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(212..230)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     480..499
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     674..697
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 accgtcagat ccgctagcat gctgctatcc gtgccgttgc tgctcggcct cctcggcctg
       61 gccgtcgccg accggtcgat ggtgagcaag ggcgaggagg tcatcaagga gttcatgcgc
      121 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      181 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      241 ctgcccttcg cctgggacat cctgtcccct cagtgcatgt acggctccaa gggctacgtg
      301 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      421 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      481 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      541 gacggcgccc tgaagggcga gatgaagatg aggctgaagc tgaaggacgg cggccactac
      601 gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc cggcgcctac
      661 aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
      721 tacgagcgcg ccgagggccg ccactccacc ggcgccaagg acgagctgta agaattctgc
      781 agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca
      841 taccacattt gtagag
//
