LOCUS       sequence-134600-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ATF4_1
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..44
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             51..71
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
       61 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
      121 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
      181 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
      241 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
      301 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
      361 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
      421 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
      481 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
      541 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
      601 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
      661 agacctacaa ccagctgttc gaggaa
//
