LOCUS       sequence-134601-         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_ATF4_2
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        110..358
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     110..130
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     281..300
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        379..454
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtg agggcctatt
      121 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      181 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtanaa agtaataatt
      241 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      301 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc
      361 tcctgactat cctcaactgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
      421 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gtttta
//
