LOCUS       sequence-134609-         512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 512)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PX458_HHEX_2
FEATURES             Location/Qualifiers
     source          1..512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        109..358
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     109..129
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        380..455
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtga gggcctattt
      121 cccatgattc cttcatattt gcatatacga tacaaggctg attagagaga taattggaat
      181 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtaaaa agtaataatt
      241 tcttgggtag tttgcanttt taaaattatg ttttaaaatg gactatcnta tgcttaccgt
      301 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgt
      361 ctgaacatgc caatgccagg ttttagagct agaaatagca agttaaaata aggctagtcc
      421 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag ctagaaatag
      481 caagttaaaa taaggctagt ccgtttttag cg
//
