LOCUS       sequence-134610-        5580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LTR7-driving reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Wang J, Xie G, Singh M, Ghanbarian AT, Rasko T, Szvetnik A, Cai H, 
            Besser D, Prigione A, Fuchs NV, Schumann GG, Chen W, Lorincz MC, 
            Ivics Z, Hurst LD, Izsvak Z
  TITLE     Primate-specific endogenous retrovirus-driven transcription defines 
            naive-like stem cells.
  JOURNAL   Nature. 2014 Dec 18;516(7531):405-9. doi: 10.1038/nature13804. Epub 
            2014 Oct 15.
  PUBMED    25317556
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13804"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "405-9"
COMMENT     Sequence Label: pT2/LTR7-GFP
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1159..1824
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             1159..1824
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     primer_bind     complement(1838..1857)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    1867..1948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1892..1911
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2423..2441)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2462..2478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2462..2478)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2475..2497)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2486..2502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2510..2516,2517..2534,2535..2540))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2555..2576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2693..2710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2864..3452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2944..2963)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3623..4414,4415..4483))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4246..4265
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4484..4588)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4615..5070
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4702..4721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4912..4933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5196..5218
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5210..5227
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5211..5227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5237..5256
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5237..5255
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaattcatgc tgcgagatgg gaaacacata caaaatcttc aaccttcagt aagtaaaaac
       61 cttctctatt aaaatctgca aagtgtattc atttgttcta aaattatttg ctaagtgccc
      121 acacagcact aggaatgaaa cataaaaaaa tctcttccct cacttagctt cgtattctct
      181 ttgggaatgt caggcctctg agcccaagcc aagccatcgc atcccctatg acatgcacgt
      241 acacgcccag atggcctgaa gtaactgaag aatcacaaaa gaagtgaata tgccctgccc
      301 caccttaact gatgacattc caccacaaaa gaagtgtaaa tggccagtcc ttgccttaac
      361 tgatgacatt accttgtgaa agtccttttc ctggctcatc ctggctcaaa aagcaccccc
      421 actgagcacc ttgcgacccc ccgctcctac ccgccagaga acaaaccccc tttgactgta
      481 attttccttt acctaaccaa atcctataaa acggccccac ccttatctcc cttcgctgac
      541 tctcttttcg gactcagccc gcctgcaccc aggtgaaata aacagcctcg ttgctcacac
      601 aaagcctgtt tggtggtctc ttcacacgga cgcgcatgaa atttggtgcc gtgactcgga
      661 tcgggggacc tcccttggga gatcaatccc ctgtcctcct gctctttgct ccgtgagaaa
      721 gatccaccta cgacctcagg tcctcagacc aaccagccca agaaacatct caccaatttc
      781 aaatccggta agcggcctct ttttactctg ttctccaacc tccctcacta tccctcaacc
      841 tctttctcct ttcaatcttg gcgccacact tcaatctctc ccttctctta atttcaattc
      901 ctttcattct ctggtagaga caaaagagac atgttttatc cgtgaaccca aaactccggc
      961 gccggtcacg gactgggaag gcagtcttcc cttggtgttt aatcattgca gggacgcctc
     1021 tctgatttca cgtttcagac cacgcaggga tgcctgcctt ggtccttcac ccttagcggc
     1081 aagtcccgct ttcctggggc aggggcaagt acccctcaac cccttctcct tcacccgcta
     1141 gcgctaccgg tcgccaccat gcccgccatg aagatcgagt gccgcatcac cggcaccctg
     1201 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccgagca gggccgcatg
     1261 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     1321 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     1381 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     1441 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     1501 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     1561 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     1621 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     1681 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     1741 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     1801 ttcaagaccc ccatcgcctt cgccagatct cgagctcgat gagtttggac aaaccacaac
     1861 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1921 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1981 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtac
     2041 ttaagaggga tcgataagga tctagcttgt ggaaggctac tcgaaatgtt tgacccaagt
     2101 taaacaattt aaaggcaatg ctaccaaata ctaattgagt gtatgtaaac ttctgaccca
     2161 ctgggaatgt gatgaaagaa ataaaagctg aaatgaatca ttctctctac tattattctg
     2221 atatttcaca ttcttaaaat aaagtggtga tcctaactga cctaagacag ggaattttta
     2281 ctaggattaa atgtcaggaa ttgtgaaaaa gtgagtttaa atgtatttgg ctaaggtgta
     2341 tgtaaacttc cgacttcaac tgtataggga tcctctagct agagtcgacc tcgagggggg
     2401 gcccggtacc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
     2461 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2521 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     2581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3301 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3481 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     4621 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4681 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4741 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4801 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4861 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4921 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4981 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5041 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
     5101 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     5161 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     5221 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcg gatccctata
     5281 cagttgaagt cggaagttta catacactta agttggagtc attaaaactc gtttttcaac
     5341 tactccacaa atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact
     5401 ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata
     5461 attcactgta tcacaattcc agtgggtcag aagtttacat acactaagtt gactgtgcct
     5521 ttaaacagct tggaaaattc cagaaaatga tgtcatggct ttagaagctt gatatccatg
//
