LOCUS       sequence-134616-         429 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing FKBPF36V protein in washing out shield-1 ligand for 
            FKBPdd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Egeler EL, Urner LM, Rakhit R, Liu CW, Wandless TJ
  TITLE     Ligand-switchable substrates for a ubiquitin-proteasome system.
  JOURNAL   J Biol Chem. 2011 Sep 9;286(36):31328-36. doi: 
            10.1074/jbc.M111.264101. Epub 2011 Jul 15.
  PUBMED    21768107
REFERENCE   2  (bases 1 to 429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.264101"; journalName: "J Biol Chem"; date: 
            "2011-09-9- 9"; volume: "286"; issue: "36"; pages: "31328-36"
COMMENT     Sequence Label: pET15b His6-FKBPF36V
FEATURES             Location/Qualifiers
     source          1..429
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(97..99,100..423)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggaggtgga ggatccatgg gagtgcaggt ggaaaccatc
      121 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc
      181 gggatgcttg aagatggaaa gaaagttgat tcctcccggg acagaaacaa gccctttaag
      241 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
      301 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
      361 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa
      421 tgaggatcc
//
