LOCUS       sequence-134628-         551 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial express ecDHFR protein for washing out TMP ligand for pBMN
            DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid 
            #29326).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 551)
  TITLE     Wandless lab ecDHFR plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b His6-ecDHFR (WT)
FEATURES             Location/Qualifiers
     source          1..551
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             42..59
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             66..545
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 ccatgggcag cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc
       61 atatgatgat cagtctgatt gcggcgttag cggtagatcg cgttatcggc atggaaaacg
      121 ccatgccgtg gaacctgcct gccgatctcg cctggtttaa acgcaacacc ttaaataaac
      181 ccgtgattat gggccgccat acctgggaat caatcggtcg tccgttgcca ggacgcaaaa
      241 atattatcct cagcagtcaa ccgggtacgg acgatcgcgt aacgtgggtg aagtcggtgg
      301 atgaagccat cgcggcgtgt ggtgacgtac cagaaatcat ggtgattggc ggcggtcgcg
      361 tttatgaaca gttcttgcca aaagcgcaaa aactgtatct gacgcatatc gacgcagaag
      421 tggaaggcga cacccatttc ccggattacg agccggatga ctgggaatcg gtattcagcg
      481 aattccacga tgctgatgcg cagaactctc acagctattg ctttgagatt ctggagcggc
      541 ggtaaggatc c
//
